We purified and characterized a soluble human interferon gamma receptor expressed in Escherichia coli. The soluble receptor comprises the amino acids 15-246 of the encoded protein (Aguet, M., Dembic, Z., and Merlin, G. (1988) Cell 55, 273-280) and was purified from large scale fermentations through four chromatographic steps with an overall recovery of 28%. The refolded soluble receptor shows s...

background: the acute phase response is beneficial to theanimal in restoring homeostasis, and measuring the circulatingacute phase proteins, cytokines, and enzymes can be used toevaluate the innate immune system's responses to invader agentssuch as bacterial lipopolysaccharide. measurement of theseparameters has shown to be useful as diagnostic and prognosticmarkers in animal endotoxemia. objec...

Keratinocytes are routinely subjected to both internal and external stimulation. This study investigates the effects of interferon gamma, interleukin-4, tumor necrosis factor alpha, and the synthetic immunomodulator muramyl dipeptide on the human keratinocyte cell line, HaCaT. Following HaCaT stimulation with cytokines or muramyl dipeptide for different time periods, changes in the expression o...

PD-L1 and PD-L2 are ligands for the PD-1 immune inhibiting checkpoint that can be induced in tumors by interferon exposure, leading to immune evasion. This process is important for immunotherapy based on PD-1 blockade. We examined the specific molecules involved in interferon-induced signaling that regulates PD-L1 and PD-L2 expression in melanoma cells. These studies revealed that the interfero...

Objective: The interleukin-12/interferon-γ (IL-12/IFN-γ) pathway is the most validated cytokine regulating Mycobacterium tuberculosis infection. role of IL-12/IFN-γ axis in protecting against (TB) exhibited people having mutations genes encoding these cytokines. We aimed to study serum levels IL-12 and IFN-γ pediatric their correlation with clinical microbiological features. Material Methods: A...

interleukin-4 (il-4) is produced by t-helper cells type 2 (th2) and induces ige synthesis. t-helper cells type 1 (th1) produce interferon-gamma (ifn-gamma) which suppresses th2 and reduces il-4 induced ige production. in this :;tudy , we demonstrated that the levels of specific ige in the serum of atopic children (n=20) were elevated while il-4 production was increased and ifn-gamma secretion w...

Background and Aims: chronic HBV infection is one of the most common viral infections in worldwide which many factors such as genetic factors are involved in pathogenesis of disease. Gamma interferon (IFN-γ) and its receptor (INFGR) play a critical role in the immune response to HBV infection. Single nucleotide polymorphisms (SNPs) are effective on level of gene expression, The aim of thi...

BACKGROUND: The acute phase response is beneficial to theanimal in restoring homeostasis, and measuring the circulatingacute phase proteins, cytokines, and enzymes can be used toevaluate the innate immune system's responses to invader agentssuch as bacterial lipopolysaccharide. Measurement of theseparameters has shown to be useful as diagnostic and prognosticmarkers in animal endotoxemia. OBJEC...