The nervous system plays an important but poorly understood role in modulating longevity. GABA, a prominent inhibitory neurotransmitter, is best known to regulate nervous system function and behaviour in diverse organisms. Whether GABA signalling affects aging, however, has not been explored. Here we examined mutants lacking each of the major neurotransmitters in C. elegans, and find that defic...

The diterpene lactones of Ginkgo biloba, ginkgolides A, B and C are antagonists at a range of Cys-loop receptors. This study examined the effects of the ginkgolides at recombinant human ρ(1) GABA(C) receptors expressed in Xenopus oocytes using two-electrode voltage clamp. The ginkgolides were moderately potent antagonists with IC(50)s in the μM range. At 10 μM, 30 μM and 100 μM, the ginkgolides...

BACKGROUND Multiple excitatory neurotransmitters have been identified in the mammalian taste transduction, with few studies focused on inhibitory neurotransmitters. Since the synthetic enzyme glutamate decarboxylase (GAD) for gamma-aminobutyric acid (GABA) is expressed in a subset of mouse taste cells, we hypothesized that other components of the GABA signaling pathway are likely expressed in t...

The loop C hydrophilic residue, threonine 244 lines the orthosteric binding site of ρ1 GABAC receptors was studied by point mutation into serine, alanine and cysteine, and tested with GABA, some representative partial agonists and antagonists. Thr244 has a hydroxyl group essential for GABA activity that is constrained by the threonine methyl group, orienting it toward the binding site. Signific...

introduction: the aim of the present study is to investigate the effect of interaperitoneal injection of baclofen (gaba-b agonist) and cgp35348 (gaba-b antagonist) on serum testosterone concentration in aggressive rats exposed to electromagnetic field. methods: fifty five mature male rats weighing 200±20 grams were studied. animals were divided into 2 main groups and four subgroups. main groups...

We investigated the ubiquitin-like modification of GABA(A) receptor-associated protein (GABARAP) and its function. A fusion protein of GABARAP with v5 in the N terminus and myc in the C terminus was expressed in rat cultured hippocampal neurons and PC12 cells. Western blotting with antibodies to v5 and myc revealed that the C terminus of GABARAP was cleaved off. Cleavage was blocked by mutating...

in this paper it has been shown that deterioration of the brain cortex capillary system and negative dynamics of cerebral tissue morphology occur under conditions of hypo kinesia. simultaneously, gamma-aminobutyric acid (gaba) and piracetam have been shown to favor the development of vasodilation and prevent further worsening of the cerebral blood supply. during the experiment, it was also esta...

GABA(B) receptor subtypes are based on the subunit isoforms GABA(B1a) and GABA(B1b), which associate with GABA(B2) subunits to form pharmacologically indistinguishable GABA(B(1a,2)) and GABA(B(1b,2)) receptors. Studies with mice selectively expressing GABA(B1a) or GABA(B1b) subunits revealed that GABA(B(1a,2)) receptors are more abundant than GABA(B(1b,2)) receptors at glutamatergic terminals. ...

In this paper it has been shown that deterioration of the brain cortex capillary system and negative dynamics of cerebral tissue morphology occur under conditions of hypo kinesia. Simultaneously, gamma-aminobutyric acid (GABA) and piracetam have been shown to favor the development of vasodilation and prevent further worsening of the cerebral blood supply. During the experiment, it was also ...

Introduction The GABAergic system is complex; GABA may act at multiple subtypes of ionotropic and metabotropic GABA receptors, at GABA transporters and it may be metabolized in distinct metabolic pools of GABA. Discriminating this plethora of activities is problematic. Here, we describe a novel approach where the system is perturbed by specific GABA receptor ligands with known (or partially kno...