The aim of the experiment was to histochemically examine the activity of the following selected steroidogenic enzymes: delta5 3betaHSD and 17betaHSD and the housekeeping enzyme G6PDH, in experimental rats after subcutaneous injections of naringenin flavonoid, at a daily dose of 15 mg/kg of body mass. The enzyme activities were measured by the microdensitometric method. Additionally, radioimmuno...

A convenient homogeneous enzyme immunoassay for estradiol is described. Unlike heterogeneous immunoassays, which require time-consuming separation steps or expensive automated systems, homogeneous immunoassays, wherein all reagents are freely suspended in bulk solution, can be simple and fast without costly instrumentation. The key component of this assay system, an estradiol-reporter enzyme co...

Enzymatic and histopathologic alterations of the digestive gland, gill, gonad, and kidney were studied in Asian clam (Potamocorbula amurensis) in April, 1997 from each of four United States Geological Survey (USGS) stations in the San Francisco Estuary. Stations were selected based on differing body burdens of metallic contaminants in clams (Stn 4.1> 6.1> 8.1>12.5) observed over 7 year...

Little is known about the way in which carnivorous fish such as salmonids mobilise and metabolise dietary carbohydrates, which are essential to lipid metabolism. Thus we have studied changes caused by the absence of dietary carbohydrates to the kinetics and molecular behaviour of the four cellular NADPH-production systems [glucose 6-phosphate dehydrogenase (G6PDH); 6-phosphogluconate dehydrogen...

The effects ofthe testicular feminization (Tfm) mutation on androgen metabolism, binding, and action were studied in the mouse. Cytosolic binding of ['H] dihydrotesterone (DHT) was reduced by approximately 90% in the brains, pituitaries, submaxillary glands, and kidneys ofTfmIY males, as compared to wild-type male controls. N uclear ['H] DHT binding was abolished in tissues from Tfm male animal...

Background. The radioresistance of glioma stem cells (GSCs) is related to some microRNAs (miRs) generated by radiation. This study aimed investigate the effects miR-17-5p and miR-130b-3p on radiosensitivity GSCs. Methods. expressions in SU3 SU3-5R were determined. transfected with or mimics inhibitors used determine cell viability after irradiation as well examine changes supernatant glucose, i...

Glucose 6-Phosphate Dehydrogenases (G6PDHs) from different sources show varying specificities towards NAD+ and NADP+ as cofactors. However, it is not known to what extent structural determinants of cofactor preference are conserved in the G6PDH family. In this work, molecular simulations, kinetic characterization of site-directed mutants and phylogenetic analyses were used to study the structur...

OBJECTIVE We investigated whether estradiol may prevent accelerated atherosclerosis due to hyperhomocysteinemia by enhancing the antioxidant system. METHODS Male Wistar rats were treated with placebo (P) or 1 mg (1E2) and 2 mg (2E2) 17 beta estradiol. Half of the animals (n=6) from each group received homocysteine (Hcy, 100 mg/kg/day) administered in the drinking water for 60 days (P/Hcy, 1E2...

This study examined the effects of corn gluten (CG) and its hydrolysate consumptions on weight reduction in rats fed a high-fat diet. Eight-month-old male Sprague-Dawley rats (n=40) were fed a high-fat diet (40% calorie as fat) for 4 weeks. They were then randomly divided into four groups and fed the isocaloric diets with different protein sources for 8 weeks. The protein sources were casein (c...