Introduction While conception rates following cervical insemination of synchronised ewes with fresh semen are generally around 60-70%, conception rates with frozen-thawed semen are unacceptably low (<20% generally). These poor conception rates following cervical insemination with frozen-thawed semen appear to stem from two inter-related factors which are additive in their negative impacts: (a) ...

A total of 30 ejaculates from three Hampshire crossbred boars were used to study the effect of freezing rates and thawing methods on quality of frozen boar semen. Semen was frozen using three freezing rates-30 0 c/min, 50 0 c/min and 70 0 c/min in a programmable biological semen freezer. The semen characteristics viz., sperm motility, live sperm, HOST reacted spermatozoa and intact acrosome wer...

This article covers recent developments in the field of equine artificial insemination. Use of both chilled and frozen semen is discussed. For chilled semen, there is new information about what to do if the semen shipment is delayed and on maximising pregnancy rates in older mares by careful evaluation of the cervix. The number of motile sperm needed for insemination is reviewed in light of the...

Introduction Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1780, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum condition...

There has and will continue to be reproductive techniques available that have a positive impact upon the equine breeding industry. This review focuses on semen technologies that have been developed or are in the process of being developed. The use of fluorescent dyes and flow cytometry has provided the researcher and clinician with powerful tools to evaluate several sperm attributes. These proc...

AIM To evaluate different thawing temperatures and duration on the post-thaw semen quality of Indian yaks bulls. MATERIALS AND METHODS Semen ejaculates from four different yak bulls were collected using artificial vagina method and extended with tris extender containing 6.4% glycerol at 35°C, cooled gradually from 35°C to 5°C at 1°C/3 min and equilibrated at 4-5°C for 4 h and frozen in French...

The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...

BACKGROUND Although routinely used in assisted reproductive technology, human sperm cryopreservation is not an entirely successful procedure. This study determined the effect of nerve growth factor (NGF) supplementation of cryopreservation medium on post-thaw viability, motility, intracellular nitric oxide (NO) concentration, and DNA fragmentation of human spermatozoa in asthenozoospermic men. ...

Methods of collection and freezing of semen of some deer species and aspects of controlled reproduction associated with the use of frozen-thawed semen by artificial insemination (AI) are discussed.

Two hours after semen collection, aliquots containing 6xlO 9 spermatozoa were centrifuged for 10 rain at 300 g. The seminal plasma was removed and the spermatozoa were resuspended to 5 ml with Beltsville F5 (BF5) extender. The semen was cooled gradually to 5 C over a 2-hr period, 5 ml of BF5 containing 2% glycerol was added and the semen was mixed and frozen immediately into pellets of 0.15 to ...