Cold temperatures trigger the expression of the CBF family of transcription factors, which in turn activate many downstream genes that confer chilling and freezing tolerance to plants. We report here the identification of ICE1 (inducer of CBF expression 1), an upstream transcription factor that regulates the transcription of CBF genes in the cold. An Arabidopsis ice1 mutant was isolated in a sc...

To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (Fragaria × ananassa) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('Jonsok') and least-tolerant cultivar ('Frida') were compared with a go...

Temperate plants are capable of developing freezing tolerance when they are exposed to low nonfreezing temperatures. Acquired freezing tolerance involves extensive reprogramming of gene expression and metabolism. Recent full-genome transcript profiling studies, in combination with mutational and transgenic plant analyses, have provided a snapshot of the complex transcriptional network that oper...

Cold acclimation is an important adaptive response of plants from temperate regions to increase their freezing tolerance after being exposed to low nonfreezing temperatures. The three CBF genes are well known to be involved in cold acclimation. As the 3 CBF genes are linked tandemly in the Arabidopsis genome, it is almost impossible to obtain cbf triple mutants using traditional genetic methods...

doi: 10.1111/j.1399-3054.2007.00904.x Big sagebrush (Artemisia tridentata) is a dominant shrub throughout much of the arid western United States. Several recognized subspecies differ in physiology, morphology and in their distribution in relation to soil water availability. While several studies have compared mature individuals of these subspecies, there is little information on seedling physio...

BACKGROUND AND AIMS Cold is a major constraint for cereal cultivation under temperate climates. Winter-hardy plants interpret seasonal changes and can acquire the ability to resist sub-zero temperatures. This cold acclimation process is associated with physiological, biochemical and molecular alterations in cereals. Brachypodium distachyon is considered a powerful model system to study the resp...

The CBF (C-repeat binding factor) pathway has a major role in plant cold acclimation, the process whereby certain plants increase in freezing tolerance in response to low nonfreezing temperatures. In Arabidopsis thaliana, the pathway is characterized by rapid cold induction of CBF1, CBF2, and CBF3, which encode transcriptional activators, followed by induction of CBF-targeted genes that impart ...

Assessing freezing community response and whether freezing resistance is related to other functional traits is essential for understanding alpine community assemblages, particularly in Mediterranean environments where plants are exposed to freezing temperatures and summer droughts. Thus, we characterized the leaf freezing resistance of 42 plant species in 38 plots at Sierra de Guadarrama (Spain...

doi: 10.1111/j.1399-3054.2005.00596.x Cold acclimation involves precise signaling and regulation of the transcriptome. The plasma membrane may be the primary cold-stress sensor, and FRY1/HOS2 inositol polyphosphate 1-phosphatase regulates cytosolic inositol-1,4,5-triphosphate levels, which in turn control cytosolic Ca2þ signatures and cold acclimation. Cold-induced reactive oxygen species may a...

Brassica napus suspension-cultured cells can be hardened to a lethal temperature for 50% of the sample of -20 degrees C in eight days at room temperature with abscisic acid. During the induction of freezing tolerance, changes were observed in the electrophoretic pattern of [(35)S]methionine labeled polypeptides. In hardening cells, a 20 kilodalton polypeptide was induced on day 2 and its level ...