The history of IVF is synonymous with several millstones and each of these have led to a revolution in our understanding of human fertility or causes of male and female infertility, as well as introducing new treatment protocols for infertility. It can undoubtedly be claimed that one of the best of these was the introduction of ultrarapid glass-like solidification of a solution without ice-crys...

The chemical stability of repository compounds is affected by various environmental conditions during long-term storage. Studies were carried out to evaluate the effects of the following potential causes of instability of compounds in DMSO at a 10-mM concentration: water, oxygen, freeze/thaw cycles, and storage container material. A set of compounds was selected for the study based on structura...

OBJECTIVE To evaluate and compare the laboratory and clinical outcomes of vitrification with slow-freezing method for cryopreservation of embryos and blastocysts in an in-vitro fertilisation programme. DESIGN Retrospective analysis of all the 104 cycles of frozen embryo and blastocyst replacements from 2003 to 2008 and all the 149 cycles with embryos or blastocysts for vitrification from 2006...

Previous study demonstrated that the low survival of human embryonic stem cells (hESC) under conventional slow-cooling cryopreservation protocols is predominantly due to apoptosis rather than cellular necrosis. Hence, this study investigated whether a synthetic broad-spectrum irreversible inhibitor of caspase enzymes, Z-VAD-FMK can be used to enhance the post-thaw survival rate of hESC. About 1...

Most previous studies of the Qinghai-Tibet engineering corridor (QTEC) have focused on the impacts of climate change on thaw-induced slope failures, whereas few have considered freeze-induced slope failures. Terrestrial laser scanning was used in combination with global navigation satellite systems to monitor three-dimensional surface changes between 2014 and 2015 on the slope of permafrost in ...

Loess, a collapsible soil, is widely distributed in the Qinghai–Tibet Plateau China. In meantime, loess sustainable and green building material that used traditional residential buildings. However, previous studies have focused on properties of itself, ignoring influence climatic conditions buildings, as well series engineering problems such soil spalling caused by freezing thawing, cold cracks...

BACKGROUND Trehalose is related to several types of stress responses, especially freezing response in baker's yeast (Saccharomyces cerevisiae). It is desirable to manipulate trehalose-related genes to create yeast strains that better tolerate freezing-thaw stress with improved fermentation capacity, which are in high demand in the baking industry. RESULTS The strain overexpressing MAL62 gene ...

This paper presents an experimental investigation to identify suitable indices assess durability of glulam when subjected freeze-thaw cycles in exposed enviroenment. In this study, two types specimens were tested for their performance different levels aging due freezing and thawing. Effect treatment on various parameters including thickness swelling rate, static bending strength, elastic modulu...

In regions exposed to floods followed by cold weather, brick masonry as a structural basis of the building envelope can be damaged due accompanying phenomenon freezing and thawing. The main purpose article is development mathematical model able predict chosen mechanical parameters damage index wallets for given number freeze-thaw cycles. For this, statistical derived from experimental data used...

Freezing of eggs or ovarian tissue to preserve fertility for cancer patients has been studied since 1994 with Gosden’s original paper in sheep, and before that in smaller mammals for decades prior. Clinically, this tactic has enjoyed great success. Despite the rising popularity of vitrification for egg freezing, it is often done incorrectly, forgetting the original principles Kuwayama establish...