BACKGROUND Circulating microRNAs are potential markers for disease detection. A novel class of small non-coding RNAs called Piwi-interacting RNAs (piRNAs) has been recently reported to participate in the epigenetic regulation of cancers and other diseases. This study aims to discover blood-based piRNAs which can be used as markers for disease detection and monitoring. MATERIALS AND METHODS We...

Freeze-thaw cycles induce major hydraulic changes due to liquid-to-ice transition within tree stems. The very low water potential at the ice-liquid interface is crucial as it may cause lysis of living cells as well as water fluxes and embolism in sap conduits, which impacts whole tree-water relations. We investigated water fluxes induced by ice formation during freeze-thaw cycles in Juglans reg...

The Amplicor HIV-1 Monitor test was compared to the nucleic acid sequence-based amplification (Nasba) assay system for the quantitation of human immunodeficiency virus (HIV) RNA in three different types of clinical samples: plasma, serum, and plasma subjected to freeze-and-thaw cycles. Each assay detected HIV RNA in the same 73 (90%) of 81 samples tested, and the quantitative results obtained w...

To investigate the dynamic compressive properties and law of energy dissipation freeze–thaw-damaged sandstone, static experiments were conducted. The influences number freeze–thaw cycles strain rate on strength characteristics, fractal dimension characteristics sandstone evaluated. Based peak rate, a damage variable was established. results show that increases exponentially with there exists th...

OBJECTIVE(S) To determine if mishandling prior to testing would make a sample from a chronically infected subject appear recently infected when tested by cross-sectional HIV incidence assays. METHODS Serum samples from 31 subjects with chronic HIV infection were tested. Samples were subjected to different handling conditions, including incubation at 4 °C, 25 °C and 37 °C, for 1, 3, 7 or 15 da...

According to standards, the durability performance of fiber-cement sheets must be evaluated by comparing the modulus of rupture (MOR) before and after durability tests (freeze-thaw, soak-dry, and warm water) are completed. This paper investigated the MOR of two different fiber-cement sheets samples before and after durability tests as well as its ductility and toughness. Results showed no signi...

Pressure transducers were attached to twigs of orchard trees and potted trees of walnut (Juglans regia L.) to measure winter stem xylem pressures. Experimental potted trees were partially defoliated in the late summer and early autumn to lower the amount of stored carbohydrates. Potted trees were placed in cooling chambers and subjected to various temperature regimes, including freeze-thaw cycl...

As an important type of soil erosion, freeze-thaw erosion occurs primarily at high latitude and altitude. The overview on the effect was provided. Soil affected by processes, as thawing water reinforce each other. Remote sensing provided unprecedented approach for characterizing timing, magnitude, patterns large-scale changes. Furthermore, essence freeze thaw moisture in pores soil. Freeze-thaw...

Relationships between abiotic (soil temperature and number of freeze-thaw cycles) or biotic factors (chemical elements, microbial biomass, extracellular enzymes, and decomposer communities in litter) and litter decomposition rates were investigated over two years in subalpine forests close to the Qinghai-Tibet Plateau in China. Litterbags with senescent birch, fir, and spruce leaves were placed...

BACKGROUND Methane (CH(4)) uptake by steppe soils is affected by a range of specific factors and is a complex process. Increased stocking rate promotes steppe degradation, with unclear consequences for gas exchanges. To assess the effects of grazing management on CH(4) uptake in desert steppes, we investigated soil-atmosphere CH(4) exchange during the winter-spring transition period. METHODOL...