In this study we heated insoluble residues, obtained after Triton-X-100 (0.1 v/v%) extraction of erythrocyte ghost membranes (EGMs). Specific heat capacity, electric capacitance and resistance, and optical transmittance (280 nm) sustained sharp changes at 49°C (TA) and 66°C (TC), the known denaturation temperatures of spectrin and band 3, respectively. The change at TA was selectively inhibited...

A diverse set of 320 compounds from the Procter & Gamble Pharmaceuticals organic compound repository was prepared as 20-mM DMSO solutions and stored at 4 degrees C under argon in pressurized canisters to simulate a low-humidity environment. The plates were subjected to 25 freeze/thaw cycles while being exposed to ambient atmospheric conditions after each thaw to simulate the time and manner by ...

Plasma membrane ATPase has been proposed to be functionally altered during early stages of injury caused by a freeze-thaw stress. Complete recovery from freezing injury in onion cells during the postthaw period provided evidence in support of this proposal. During recovery, a simultaneous decrease in ion leakage and disappearance of water soaking (symptoms of freeze-thaw injury) has been noted....

The Amplicor HIV-1 Monitor test was compared to the nucleic acid sequence-based amplification (Nasba) assay system for the quantitation of human immunodeficiency virus (HIV) RNA in three different types of clinical samples: plasma, serum, and plasma subjected to freeze-and-thaw cycles. Each assay detected HIV RNA in the same 73 (90%) of 81 samples tested, and the quantitative results obtained w...

In the last decade, an increasing number of studies have reported that soil nitrous oxide (N2O) emissions can be reduced by adding biochar. However, the effect of biochar amendment on soil N2O emissions during freeze-thaw cycle (FTC) is still unknown. In this laboratory study, biochar (0%, 2% and 4%, w/w) was added into a cultivated sandy loam soil and then treated with 15 times of FTC (each FT...

Freezing and thawing of soils may affect the turnover of soil organic matter and thus the losses of C and N from soils. Here we review the literature with special focus on: (i) the mechanisms involved, (ii) the effects of freezing temperature and frequency, (iii) the differences between arable soils and soils under natural vegetation, and (iv) the hypothesis that freeze-thaw events lead to sign...

The investigation involved twelve different synthetic aggregates produced in the TTl rotary kiln, one commercially produced synthetic lightweight aggregate, and one regular weight aggregate. Each aggregate was used in concrete which was subjected to a variety of physical durability investigations including autoclave expansion, long-time reactivity, freeze-thaw, and shrinkage. From the results o...

Cytokines are a group of peptides which form a sophisticated network to modulate multiple cellular events. Within such a network, and through complex feedback mechanisms, cytokine functions are largely interdependent and closely associated with a number of pathological processes. In the present study, the EVIDENCE 180 system was used to study the ...

R. P. Goodrich and co-workers, 1989 have reported that red blood cells (RBCs) can be preserved in the dry state by addition of mixtures of hydroxyethyl starch (HES) and glucose (59,60). In this thesis work we tried to investigate the effect of HES alone on the viability, functionality and oxidative state of red blood cells after freeze thaw stress. Here we prepared eight formulations having var...