Hemolytic anemia caused by oxidative drugs is thought to result from the oxidation of intracellular and membrane sulfhydryl groups of the erythrocyte. This process is more likely to occur in those erythrocytes in which the intracellular mechanism for reduction of disulfides is abnormal (e.g., glucose-6-phosphate dehydrogenase deficiency). If a membrane sulfhydryl group is critical in the pathog...

Effects of sulfhydryl modification on the ATP regulated K+ channel (KATP channel) in the pancreatic beta-cell were studied, using the patch clamp technique. Application of the sulfhydryl oxidizing agents thimerosal and 2,2'-dithio-bis(5-nitropyridine) (DTBNP), in micromolar concentrations, caused complete inhibition of the KATP channel, in inside-out patches. The inhibition was rapid and was re...

Certain reducing agents containing sulfhydryl groups are important to the in-vitro survival of Treponema pallidum. Discrepancies occur, however, concerning the agents and the concentrations that are optimal. To clarify some of this confusion, sulfhydryl oxidation was determined using procedures and experimental conditions commonly used for T pallidum. Sulfhydryl oxidation varied according to th...

conclusions allicin vapor, or some decomposition product of allicin, causes inhibition of bacterial growth and biofilm formation. parallel enzymatic studies confirmed this inhibitory effect and suggest that sulfhydryl enzymes are involved in biofilm formation in the strain studied. results an inhibition effect was observed both in the bacterial cultures and the enzymatic solutions, and the exte...

This study was undertaken to investigate the effect of exogenous sulfhydryl compound administration on the toxicity of doxorubicin in mice. Pretreatment of CDF1 mice with a pharmacologic dose (2,000 mg/kg) of n-acetyl-l-cysteine 1 h before doxorubicin (20 mg/kg, i.p.) decreased lethality from 100% (n = 44) to 37.7% (n = 53), P less than 0.001. Variation in the timing and dose of n-acetylcystein...

Cereal Chem. 72(l):58-64 A dynamic rheometer was used to characterize the effect of glutathione, interchange. Addition of potassium bromate to dough resulted an increase potassium bromate, and two ascorbic acid isomers on the rheological in G'and a decrease in loss tangent. L-threoascorbic acid was rheologically properties of wheat flour doughs. During resting after mixing (dough more effective...

Since the discovery and isolation of glutathione from tissues by Hopkins (7) several methods have been elaborated for its quantitative determination in tissue extracts. The basic principle of all the methods in common use is the oxidation of the sulfhydryl grow, -SH, to the disulfide group, S-S, by a measurable amount of oxidant. Obviously these methods include the determination of sulfhydryl c...

We investigated the surface hydrophobicity index based on different fluorescence probes [1-anilinonaphthalene-8-sulfonic acid (ANS) and 6-propionyl-2-(N,N-dimethylamino)-naphthalene (PRODAN)], free sulfhydryl and disulfide bond contents, and particle size of 80% milk protein concentrate (MPC80) powders prepared by adding various amounts of NaCl (0, 50, 100, and 150 mM) during the diafiltration ...

antifungal and antibacterial activities at concentrations of 7.8 to 15,6uglml, respectively, and that a possible target of the ITC compounds might be the cellular sulfhydryl group, In recent years, a number of multidrug transporters have been found in various organisms including bacteria and human cells.3) A promising way to conquer such multidrug resistance is to inactivate the eMux protein in...