To perform quantitative live cell imaging, investigators require fluorescent reporters that accurately report protein localization and levels, while minimally perturbing the cell. Yet, within the biochemically distinct environments of cellular organelles, popular fluorescent proteins (FPs), including EGFP, can be unreliable for quantitative imaging, resulting in the underestimation of protein l...

In this paper we show how off-line scheduling and fixed priority scheduling (FPS) can be combined to get the advantages of both the capability to cope with complex timing constraints while providing run-time flexibility. We present a method to take advantage of the flexibility provided by FPS while guaranteeing complex constraint satisfaction on periodic tasks. We provide mechanisms to include ...

Recent data from developing rats suggest that structures downstream from the amygdala are involved in the acquisition of conditioned fear-potentiated startle (FPS). The authors tested this idea in adult rats by temporarily inactivating the structure critical for FPS, the caudal pontine reticular nucleus (PnC), during fear conditioning. When the conditioned stimulus (CS) was an odor, rats displa...

Although the past few years have seen significant, empirically-based activity in modelling First Person Shooter (FPS) game traffic, there has been only limited work published that explores the underlying reasons for observed FPS traffic characteristics. As a result the generality and scalability of these empirical models is open to question. In this paper we demonstrate how a useful predictive ...

Photoactivatable fluorescent proteins (PA-FPs) are molecules that switch to a new fluorescent state in response to activation to generate a high level of contrast. Over the past eight years, several types of PA-FPs have been developed. The PA-FPs fluoresce green or red, or convert from green to red in response to activating light. Others reversibly switch between 'off' and 'on' in response to l...

Non-player characters that act as companions for players are a common feature of modern games. Designing a companion that reacts appropriately to the player’s experience, however, is not a trivial task, and even current, triple-A titles tend to provide companions that are either static in behaviour or evince only superficial connection to player activity. To address this issue we develop an ada...

Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic v...