Pathologic diagnosis requires tissue fixation for histologic and immunohistologic analysis, and formalin is routinely used for this. The disadvantage of this fixative is its inability to preserve nucleic acids. Pathologic tumor diagnosis requires extensive molecular analyses, for which formalin fixation may be not adequate. Recently, an alcohol-based fixative (molecular fixative, MF) was descri...

BACKGROUND In the changing world of clinicopathologic practice where surgeons and pathologists are faced with increasing therapeutic demands, precise demands of each group from the other have often been reduced to blames and counter-blames. This study is thus aimed at auditing the current practice of specimen handling as a means of highlighting areas where mutual best practice is required. MA...

OBJECTIVE To date, there are only few systematic reports on the quality of DNA extracted from routine diagnostic cytologic specimens. It was the aim of the present study to evaluate the ability of 50% ethanol/2% carbowax (Saccomanno fixative) to preserve bronchial secretions with high quality genomic DNA as well as to compare different DNA extraction methods. METHODS DNA was extracted from 45...

The importance of fixatives in the study of internal structures of bacteria is evidenced in papers by Murray (1953), Cassel and Hutchinson (1955), and by Robinow (1956b). The primary purpose of the use of these substances is to maintain, without distortion, the structure and material in the site in the cell where it functioned. In addition, the fixative may in some cases bring about changes in ...

The goal of fixation is to rapidly and uniformly preserve tissue in a life-like state. While placing tissue directly in fixative works well for small pieces of tissue, larger specimens like the intact brain pose a problem for immersion fixation because the fixative does not reach all regions of the tissue at the same rate (5,7). Often, changes in response to hypoxia begin before the tissue can ...

The article presents the research results of effectiveness sunflower polysaccharide extract use as a feed pellets fixative and compound ingredient that improves it's nutritional taste value. studied optimal dose introduction into fattening pigs diet was scientifically substantiated. impact feeding containing new product in combination with whey on feeds digestibility, hematological, biochemical...