Monitoring protein-protein interactions in living cells is key to unraveling their roles in numerous cellular processes and various diseases. Previously described split-GFP based sensors suffer from poor folding and/or self-assembly background fluorescence. Here, we have engineered a micro-tagging system to monitor protein-protein interactions in vivo and in vitro. The assay is based on tripart...

C YCLOSPORINE (CyA) and FK 506 are the effective immunosuppressive agents used in human organ transplantation and a variety of autoimmune diseases. 1-3 The nephrotoxicity of Cy A is well known. 4 • 5 Preliminary reports indicate the nephrotoxic nature of FK 506. though the clinical significance is yet to be established. especially in view of the favorable side effect profile that FK 506 possess...

PURPOSE To evaluate fibroblast proliferation activity of normal Tenon's capsule and of recurrent pterygia exposed in vitro to tacrolimus (FK506). METHODS A randomized prospective study was performed with 8 samples of recurrent pterygia and 6 samples of normal Tenon's capsule from the same patient with pterygium at the Faculdade de Medicina de Botucatu - UNESP. The samples were cultivated and ...

Memory extinction involves the formation of a new associative memory that inhibits a previously conditioned association. Nonetheless, it could also depend on weakening of the original memory trace if extinction is assumed to have multiple components. The phosphatase calcineurin (CaN) has been described as being involved in extinction but not in the initial consolidation of fear learning. With t...

THERE are several reports dealing with the hepatic effect of FK 506,1-4 some of which are beneficial while others are adverse. Because the liver is a major target organ that is often the site of drug toxicity, studies directed at determining whether FK 506 affects specific hepatic function and/or its histologic appearance are important. Therefore, the present study was undertaken to examine bro...

In 1987 Ochiai and coworkers presented a report on the immunosuppressive qualities of a new immunosuppressive agent, FK 506, isolated from the fermented broth of a soil fungus, Streptomyces tsukubaensis.1 Extensive in vitro studies demonstrated the effectiveness in suppressing mixed lymphocyte cultures, presumably by inhibiting 1L-2 synthesis following ailoactivation.2 The receptor for FK 506 h...

Tacrolimus (FK-506) controls organ rejection; however, arteriolar hyalinosis is a frequent complication. By use of mice lacking FK-506-binding protein-12 in endothelial cells, Chiasson and co-workers explored the contribution of endothelial cells and the transforming growth factor-β pathway to define mechanisms of hyalinosis. Absence of this binding protein removed a tonic inhibition to activat...

EFFECTS of the drugs FK 506 and CyA on lymphocyte function have been mostly studied in vitro and in vivo in animal models. 1-5 Little information is available about changes in the immune system of patients who, having undergone orthotopic liver transplantation (OL T), are treated with immunosuppressive drugs. To evaluate cellular immunity of patients treated for prolonged periods with FK 506 or...

Protein phosphorylation and dephosphorylation are believed to functionally couple neuronal activity and synaptic plasticity. Our previous results indicated that postsynaptic Ca2+/calmodulin (CaM) signaling pathways play an important role in setting synaptic strength, and calcineurin (CaN) activity limits synaptic responses during basal synaptic transmission and long-term potentiation expression...

Formal studies have not been published on the nephrotoxicity of FK 506 when the drug was used from the outset. This kind of information was sought in 101 recipients of primary livers, 24 hearts, and 3 double lungs or heart-lung. Perioperative renal dysfunction was commonly seen, which appeared to be related to FK 506 doses and plasma levels, particularly when the drug was given IV. This was rev...