BACKGROUND The aim of this study is to compare the digital images of the tissue biopsy captured with optical microscope using bright field technique under various light conditions. The range of colour's variation in immunohistochemically stained with 3,3'-Diaminobenzidine and Haematoxylin tissue samples is immense and coming from various sources. One of them is inadequate setting of camera's wh...

m To evaluate comeal sensitivity in 50% galactose-fed rats and explore its potential use in assessing diabetic keratopathy in this animal model. Mathods: Weanling male Sprague-DawleY rats were divided into two weight-matched groups and fed Purina laboratory chow (15001) plus 1) 50% alpha cellulose fiber (controls. n=9); or 2) 50% D-salactose (n= 12). Comeal sensitivity measurements were conduct...

m To evaluate comeal sensitivity in 50% galactose-fed rats and explore its potential use in assessing diabetic keratopathy in this animal model. Mathods: Weanling male Sprague-DawleY rats were divided into two weight-matched groups and fed Purina laboratory chow (15001) plus 1) 50% alpha cellulose fiber (controls. n=9); or 2) 50% D-salactose (n= 12). Comeal sensitivity measurements were conduct...

m To evaluate comeal sensitivity in 50% galactose-fed rats and explore its potential use in assessing diabetic keratopathy in this animal model. Mathods: Weanling male Sprague-DawleY rats were divided into two weight-matched groups and fed Purina laboratory chow (15001) plus 1) 50% alpha cellulose fiber (controls. n=9); or 2) 50% D-salactose (n= 12). Comeal sensitivity measurements were conduct...

m To evaluate comeal sensitivity in 50% galactose-fed rats and explore its potential use in assessing diabetic keratopathy in this animal model. Mathods: Weanling male Sprague-DawleY rats were divided into two weight-matched groups and fed Purina laboratory chow (15001) plus 1) 50% alpha cellulose fiber (controls. n=9); or 2) 50% D-salactose (n= 12). Comeal sensitivity measurements were conduct...

Background and Objective: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still an ongoing challenge in health system worldwide. Molecular detection methods including reverse transcription loop-mediated isothermal amplification (RT-LAMP) can help cutting off the transmission chain of the virus. In this study, prompt setting up of the RT-LAMP assays were investigated using ge...

Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...

Loop-mediated isothermal amplification (LAMP), a rapid nucleic acid amplification method, was developed for the clinical diagnosis of toxoplasmosis. Three LAMP assays based on the SAG1, SAG2, and B1 genes of Toxoplasma gondii were developed. The sensitivities and specificities of the LAMP assays were evaluated by comparison with the results of conventional nested PCR. The LAMP assays were highl...

A one-year field study compared the conventional 250W IR heat lamp with an energy-efficient 175W radiant heat lamp for swine farrowing operations. The energy-efficient heat lamp showed a $36 annual cash savings per unit (assuming $0.10/kWh electricity); a 1.2% absolute reduction in piglet mortality from birth to weaning (5.0±0.28% vs. 6.2±0.44%) (P<0.01); a 45% lower lamp failure rate (18±4% vs...

Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...