objective: cigarette is a rich source of oxidants and reactive nitrogen species. nitric oxide (no) in high concentration has deleterious effects on human sperm function. antioxidant defense system in seminal plasma protects spermatozoa from the attack of reactive oxygen metabolites. the purpose of this study was to determine the correlation between cigarette smoking with the no levels and the t...

BACKGROUND The human X chromosome is enriched with testis-specific genes that may be crucial for male fertility. One is the ubiquitin-specific protease 26 (USP26). Five frequent mutations have been identified: 1737G>A, 1090C > T, 370-371insACA, 494T > C and 1423C>T (with the latter three usually detected in a cluster). Their role in infertility is still controversial. This study assesses the as...

Defects in early meiotic events are thought to play a critical role in male infertility; however, little is known regarding the relationship between early meiotic events and the chromosomal constitution of human sperm. Thus, we analyzed testicular tissue from 26 men (9 fertile and 17 infertile men), using immunofluorescent techniques to examine meiotic chromosomes, and fluorescent in situ hybri...

background: reactive oxygen species (ros) are a group of free radicals that in excessive amounts have negative influence on sperm quality and function. objective: we compared ros levels in seminal plasma of infertile men with this level in healthy donors. we also determined the ros level in semen of infertile men according to the etiology of infertility, and also the effect of smoking on its le...

Inhibin is produced by Sertoli cells of the testis, provides negative feedback on FSH secretion, and may prove to be an important marker for the functioning of seminiferous tubules. The purpose of this study was to examine the relationship between the spermatogenic function of the tests of subfertile men and the plasma concentrations of inhibin. The study was carried out on twenty infertile (or...

OBJECTIVE To evaluate DNA fragmentation in spermatozoa with normal morphological appearance. DESIGN Prospective study. SETTING Academic tertiary center. PATIENT(S) Fertile, subfertile, and infertile men were studied. INTERVENTION(S) Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescein nick-end labeling assay and morphology assessment by phase contrast in ...