lysine is nutritionally essential for humans and animals. it cannot be synthesized biologically in body. children and growing animals have a high requirement of lysine, since it is needed for bone formation. therefore, it must be added to food and feed supplement to improve the protein quality. in addition, lysine has some pharmaceutical applications in the formulation of diets with balanced am...

BACKGROUND AND PURPOSE Over the last decade, Agaricus blazei Murrill has been studied and developed as a novel functional food in Japan, Korea, China, and Taiwan. Due to the low yields, the fruiting bodies of A. blazei Murrill are relatively expensive, and a cheap and stable source of A. blazei Murrill mycelium for commercial purposes is highly desirable. Culture media and conditions were inves...

Background and Objectives: Cellulase is an important enzyme with multiple applications in industries, including food, laundry, pharmaceutical, textile, pulp, paper and biofuel industries. Solid-state fermentation (SSF) is a method for cellulase production, which includes several advantages, compared to submerged fermentation. In this study, cellulase was produced by three filamentous fungi, i.e...

Changes in the metabolite profiles of Lactobacillus sakei and its growth media, based on different culture times (0, 6, 12, and 24 h), were investigated using gas chromatography-mass spectrometry (MS) and liquid chromatography-MS with partial least squares discriminant analysis, in order to understand the growth characteristics of this organism. Cell and media samples of L. sakei were significa...

Corynebacterium glutamicum is an industrially important microorganism used for amino acids production. The present work describes the production of L-lysine from Corynebacterium glutamicum IIB-C9 through submerged fermentation process. Fifteen fermentation media were screened in this study for L-lysine production. Out of these fifteen media, FM:13 was found a best one for maximum production of ...

Currently the natural crude oil depletion has been brought into awareness. This affects many industries including petrochemical manufacturing. As a result, there are many attempts to develop novel processes to produce substitutes to such petrochemical products including plastics. Polylactic acid, made from polymerization of optically pure isomer of lactic acid, is one of such examples. In addit...

In fermented products containing Bifidobacteria, factors such as organic acid concentration and b-galactosidase activity are important in the development of flavor and texture of final products. Both the process conditions and medium components have significant effects on fluctuation of such factors. The effects of whey permeate powder and yeast extract concentrations, as nitrogen sources was i...

Important biotechnological processes depend on the efficient fermentation by Saccharomyces cerevisiae yeasts of starch hydrolysates rich in maltose and maltotriose. The rate-limiting step for fermentation of these α-glucosides is the transport across the plasma membrane of the cells. In order to contribute to a better understanding of maltose and maltotriose metabolism by S. cerevisiae, we anal...

Methods to increase temperature stability of vaccines and adjuvants are needed to reduce dependence on cold chain storage. We report herein creation and application of pVEX expression vectors to improve vaccine and adjuvant manufacture and thermostability. Defined media fermentation yields of 6g/L thermostable toll-like receptor 5 agonist flagellin were obtained using an IPTG inducible pVEX-fla...

Polyhydroxyalkanoates are biopolyesters stored as reserve source of energy by a great number of prokaryotes. It is stored as intracellular granules and during normal growth, its content in the cells usually does not reach high concentrations, usually in the range of 2–10 %, but the PHB contents can reach more than 80 % of the dry biomass if growth is limited by depletion of an essential nutriti...