D-fenfluramine (d-FEN) was once widely prescribed and was among the most effective weight loss drugs, but was withdrawn from clinical use because of reports of cardiac complications in a subset of patients. Discerning the neurobiology underlying the anorexic action of d-FEN may facilitate the development of new drugs to prevent and treat obesity. Through a combination of functional neuroanatomy...

We have recently described a new helicase, the Dna2 helicase, that is essential for yeast DNA replication. We now show that the yeast FEN-1 (yFEN-1) nuclease interacts genetically and biochemically with Dna2 helicase. FEN-1 is implicated in DNA replication and repair in yeast, and the mammalian homolog of yFEN-1 (DNase IV, FEN-1, or MF1) participates in Okazaki fragment maturation. Overproducti...

Structure-specific nucleases catalyze critical reactions in DNA replication, recombination, and repair. Recently, a structure-specific endonuclease, FEN-l, has been purified and shown to cleave DNA flap structures. Here, we describe the cloning of the murine FEN-1 gene. The nucleotide sequence of FEN-I is highly homologous to the 8accharomyces cerevisiae genes YKL510 and RAD2. We show that YKLS...

The Pto kinase confers resistance in tomato to P. syringae pv. tomato strains expressing the AvrPto protein. Physical interaction of the Pto kinase and AvrPto protein in the plant cell initiates host defense responses. The recognition event between these two proteins is very specific; AvrPto does not interact with other closely related kinases, including the Fen kinase, which shares 80% amino a...

Motivation: Of the approximately 200 proteins that have been identified for the vaccinia virus genome, many are currently listed as having an unknown function, and 7 of these are also found in all other poxvirus genomes that have been sequenced. The G5R protein of vaccinia virus is included in this list, and to date, very little is known about this essential and highly conserved protein. Conven...

Peatlands cover more than 30% of the Finnish land area and impact N2O fluxes. Denitrifiers release N2O as an intermediate or end product. In situ N2O emissions of a near pH neutral pristine fen soil in Finnish Lapland were marginal during gas chamber measurements. However, nitrate and ammonium fertilization significantly stimulated in situ N2O emissions. Stimulation with nitrate was stronger th...

BACKGROUND AND PURPOSE The purpose of this study was to use hormonal responsiveness to d-fenfluramine (d-FEN) challenge as a measure of central serotonin (5-HT) function in a comparative evaluation of serotonergic abnormalities between stroke patients and healthy elderly normal subjects to test the hypothesis that stroke may be associated with diminished serotonergic functioning. METHODS Eigh...

A hierarchical excavated iron-nitrogen codoped mesoporous carbon (Ex-FeN-MC) electrocatalyst has been successfully synthesized by pyrolysis of the unique ureidopyrimidinone containing organic precursor MAMIS by using silica as the mesoporous template and iron nitrate as the source of iron. The selected organic precursor has multifunctionalities with diverse distribution of nitrogen and oxygen s...

An experiment was conducted to assess the effect of dietary supplementation of garlic (Allium sativum) and fenugreek (Trigonella foenum-graecum L.) seed powder as herbal feed additives on growth performance, carcass characteristics and economics of feeding in broilers. A total of 320 day old broiler chicks of Cobb-400 strain were divided into four treatment groups with 4 replicates of 20 chicks...

Wetlands, including peatlands, are the main source of natural methane emission. Well-defined fen microsites have different methane emissions rates, but it is not known whether the methane-producing Archaea communities vary at these sites. Possible horizontal variations of communities, in a natural oligotrophic fen, were analysed by characterizing the methanogens from two well-defined microsites...