Background Experimental process-development and optimization is expensive and time-consuming. Real optimization by means of design of experiments involves data generation before optimization can be aimed for. This can make the way from process development to process establishment even harder, since academia or start-up research facilities might not have the possibility to generate these data. F...

BACKGROUND Fungal morphology and aeration play a significant role in the growth process of Mortierella alpina. The production of microbial oil rich in arachidonic acid (ARA) in M. alpina was enhanced by using a multi-stage fermentation strategy which combined fed-batch culture with precise control of aeration and agitation rates at proper times. RESULTS The fermentation period was divided int...

[This corrects the article DOI: 10.1186/s13068-016-0466-5.].

Apple pomace extract (APE) in aerobic variable fed batch culture under standardized fermentation parameters was evaluated for the production of baker’s yeast. The substrate was fed at a flow rate of 0.39 dm h to regulate fermentable sugar concentration between 1-2 % in bioreactor. An average specific growth rate of 0.24 h and cellular yield coefficient of 0.48 g/g sugar was obtained during bake...

Software sensors have attracted great research interests due to the problem of lacking suitable and robust online sensors for key fermentation variables in fed-batch fermentation processes. In this paper, intelligent software sensors have been developed based on multivariate statistical process control methods. The software sensors not only provide real time estimation of key variables but also...

Recombinant Escherichia coli XL1-Blue harboring a high-copy-number plasmid containing the Alcaligenes eutrophus polyhydroxyalkanoate synthesis genes could efficiently synthesize poly(3-hydroxybutyrate) (PHB) in a complex medium containing yeast extract and tryptone but not in a defined medium. One of the reasons for the reduced PHB production in a defined medium was thought to be severe filamen...

A marked enhancement in the production of cell-bound phytase in Pichia anomala was achieved in cyclic fed batch fermentation (47333 UL -1 ) in comparison with that in batch (11569 UL -1 ) and fixed volume fed batch (36911 UL -1 ) fermentations in cane molasses medium. Phytase productivity was sustainable in cyclic fed batch fermentation over seven days, and therefore, this mode of fed batch fer...