Mannanases, one of the important enzyme group for industry, are produced by numerous filamentous fungi, especially Aspergillus species with different fermentation methods. The aim of this study was to show the best fermentation method of β-mannanase production for fungal growth in fermenter. Therefore, different fermentation strategies in fed-batch fermentation (suspended, immobilized cell, bio...

In this work substrate feeding rate to a fermentation of lignocellulose hydrolysate for production of ethanol has been studied. During hydrolysis of lignocellulose inhibitors are formed that effect the microorganism (here Saccharomyces cerevisiae) that ferments the sugar to ethanol in a negative way. To make the hydrolysate fermentable the inhibitors are often removed by detoxification prior to...

Abstract Background A fundamental problem associated with E. coli fermentations is the difficulty in achieving high cell densities batch cultures, attributed large part to production and accumulation of acetate through a phenomenon known as overflow metabolism when supplying enough glucose for density desired. Although fed-batch configuration standard method reducing such issues, traditional sy...

Acetone-butanol-ethanol (ABE) fermentation process is a promising bioenergy option amid rising concerns over the environmental impact of fossil fuel usage. However, commercialization ABE has been marred by challenges low product yield and titer, thereby non-competitive economics. Here, we coupled cost competitive reducing agents with controlled feeding strategy to improve both titer yield. Redu...

The feed rate of substrate during fed-batch fermentation affects many physiological variables such as specific growth rate, cell mass accumulation, substrate feed per cell, substrate feed to maintenance, carbon starvation stress, by-product formation and population segregation. These variables are known, or can be theoretically assumed, to influence protein production. Feeding strategies such a...