Acetylacetone dioxygenase from Acinetobacter johnsonii (Dke1) is a non-heme Fe2+ dependent enzyme which catalyzes the oxidative degradation of β-dicarbonyl compounds. It is a homotetramer with four active sites, each containing single metal ion. Since the active site is buried, knowledge on transport of the metal ion and reactants (products) is essential for understanding the enzyme mechanism. ...

Fe is a heavy metal that often found in water. If the level exceeds maximum limit and continuously consumed, it will cause various kinds of diseases so method needed can reduce Fe2+. In this study, Fe2+ ion adsorption was carried out using pumice which aims to determine characteristics include surface area, density porosity, ability, contact time required absorb ions. The used batch method, wit...

UNLABELLED Iron and copper toxicity has been presumed to involve the formation of hydroxyl radical (*OH) from H2O2 in the Fenton reaction. The aim of this study was to verify that Fe2+-O2 and Cu+-O2 chemistry is capable of generating *OH in the quasi physiological environment of Krebs-Henseleit buffer (KH), and to compare the ability of the Fe2+-O2 system and of the Fenton system (Fe2+ + H2O2) ...

Enzymatic browning reactions by polyphenol oxidases cause alteration of appearance, flavor and nutritive value vegetables fruits. It is one the important problems for used as salads causes lots economic losses. In this study, oxidase (PPO) from Eruca sativa was extracted characterization studies were carried out. Substrate specificity variable substrates, optimum pH, temperature, effect differe...

We hypothesized that exposure of cells to H2O2 plus Fe2+ would increase formation of cell-derived lipid peroxides that would inactivate prostaglandin H synthase, resulting in decreased prostaglandin synthesis. Therefore, we treated human endothelial cells with 0-100 microM H2O2 followed immediately by addition of 0-200 microM Fe2+. After oxidant exposure, cells were stimulated with 20 microM ar...

A sulfidogenic sludge supplemented with acetate was evaluated in the anodic chamber of microbial fuel cells (MFCs) presence sulfate (SO4-2)/Fe3+ and (SO4-2)/Fe2+ to investigate MFC performance effect iron ions on composition community since are frequently present wastewater derived from several anthropogenic activities. The current densities were up 0.025 mA/cm2 0.017 for MFCs Fe2+ Fe3+, respec...

Duodenal Fe2+ uptake is essential to body Fe2+ homeostasis, but the interaction of metals with the uptake process remains unclear. The present study compared the effects of four essential trace metals (Mn2+, Zn2+, Co2+ and Ni2+) with two toxic metals (Pb2+ and Cd2+) on Fe2+ uptake across the brush border membrane of villus-attached duodenal enterocytes. Everted rat duodenum was exposed to buffe...