The superoxide-forming activity of 27,000-g particles prepared from homogenates of zymosan-treated human neutrophils is lost if the assay is conducted in the presence of 0.045% Triton X-100. This loss in activity in the presence of detergent is prevented by 40 micron flavin adenine dinucleotide (FAD), but not by flavin mononucleotide, riboflavin, adenosine 5'-diphosphate, or adenosine 5'-monoph...

Abstract Background Rhodotorula toruloides is a robust producer of triacylglycerol owing to its fast growth rate and strong metabolic flux under conditions high cell density fermentation. However, the molecular basis fatty acid biosynthesis, desaturation regulation remains elusive. Results We present characterization four desaturase (FAD) genes in R. . Biosynthesis oleic (OA) palmitoleic (POA) ...

This article describes the development, application, and validation of a scoring index for assessment of clinical signs in cats with flea allergy dermatitis (FAD). The Scoring Index for Clinical Signs of FAD was based on the evaluation and scoring of five signs over five anatomic areas, which is similar to the psoriasis area and severity index (PASI), a commonly used scoring method in human der...

BACKGROUND The beneficial effect of Δ-17 FAD is poorly understood. The aim of this study was to investigate the protective mechanism of fatty acids against atherosclerotic (AS) damage induced by oxidized low-density lipoprotein (ox-LDL) in human umbilical vein endothelial cells (HUVECs), and to identify the molecular mechanisms involved. MATERIAL AND METHODS The ox-LDL was used to induce lipoto...

OBJECTIVE Flow associated dilatation (FAD%) and intimal media thickness are established markers of early atherosclerosis. This study aimed to compare the ability of the non-invasive measurements FAD% and intimal media thickness to predict coronary artery disease. METHODS FAD% and intimal media thickness were determined using high resolution ultrasound in 122 patients with clinically suspected...

The reconstitution of apo-glucose oxidase (apo-GOx) on single-walled carbon nanotubes (SWNTs) functionalized with the cofactor, flavin adenine dinucleotide (FAD), greatly improved electron transfer turnover rate of the redox reactions in glucose sensing with glucose sensors. The research reported here is aimed to better understand molecular details of affection of the charging SWNT to the confo...

In this work, in situ FTIR thin-layer spectroelectrochemistry has been employed at a flat mercury-based electrode to obtain, uniquely, the spectrum of the reduced form of flavin adenine dinucleotide (FAD) in neutral (pH 7) solutions. The flat mercury surface was achieved by the open-circuit amalgamation of gold and it was found to display the advantageous electrochemical properties, including t...

Membrane-bound fatty acid desaturases (FADs) are of great importance and play multiple roles in plant growth and development. In the present study, 39 full-length FAD genes, based on database searches, were identified in tetraploid upland cotton (Gossypium hirsutum L.) and were phylogenetically clustered into four subfamilies. Genomic localization revealed that 34 genes were mapped on 22 chromo...

Presenilins play essential roles in memory formation, synaptic function, and neuronal survival. Mutations in the Presenilin-1 (PSEN1) gene are the major cause of familial Alzheimer's disease (FAD). How PSEN1 mutations cause FAD is unclear, and pathogenic mechanisms based on gain or loss of function have been proposed. Here, we generated Psen1 knockin (KI) mice carrying the FAD mutation L435F or...

We developed a computational method to identify NAD- and FAD-binding sites in proteins. First, we extracted from the Protein Data Bank structures of proteins that bind to at least one of these ligands. NAD-/FAD-binding residue templates were then constructed by identifying binding residues through the ligand-binding database BioLiP. The fragment transformation method was used to identify struct...