conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

conclusions according to the results of this study, quantification of hcmv and ebv observed in this study is high in periodontal tissue samples of severe chronic periodontitis. background although studies focused mainly on the identification of periopathogenic bacteria, recent reports have suggested that various herpes viruses may also be involved in the occurrence and progression of different ...

background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...

during 2009-10, real time rt-pcr and conventional rt-pcr techniques used for detecting btvs rna in 310 blood samples. for real time and gel based rt-pcr segment-1 and segment-10 selected as conserve genes to search any btv strains. using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. sensitivity of both molecular techniques evalua...

background: with the increasing concern for health and nutrition, dietary fat has attracted considerable attention. the composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. the fatty acid synthase (fasn) and stearoyl-coa desaturase (delta-9-desaturase) (scd) genes affect fatty acid compositio...

background gastric cancer is the second leading cause of cancer-related deaths worldwide and it seems that environmental and lifestyle factors and infection with helicobacter pylori (h. pylori) have a major role in the etiology of gastric cancer. objectives the aim of this study was to investigate the presence of h. pylori dna in archival gastric tissues of patients with gastric cancer disease ...

background: preeclampsia (pe) is one of the most complex and life-threatening pregnancy disorders and is considered as a major cause of mortality among mothers and fetuses worldwide. although the exact etiology of pe is not well known several lines of evidence support an immunological etiology for pe. objective: to investigate the differences in the expression of tlrs 2, 4, 5, and 6 and a group...

The difficulties involved in detecting and enumerating Mycobacterium avium subsp. paratuberculosis (MAP) as a pathogen potentially involved in Crohn's disease (CD) are well known. This study aimed to improve this situation through the application of multiple laboratory diagnostic tests to detect and isolate this bacterium from different specimens collected from CD-patients and non-CD subjects a...