We have isolated a phage clone, F2, by panning a phage library with a CTLA4-conformation recognizing mAb (anti-CTLA4 mAb). The unique sequence of 15 amino acids with an internal disulfide bond was inserted in the gene 3 proteins of F2 phage clone (F2-g3p). We show here that 1) F2-g3p was recognized with anti-CTLA4 mAb but not with anti-CD28 mAb, and 2) F2-g3p bound to CD80 but not to CD86. The ...

Defi ciencies of Factor XI (FXID) and Uridine Monophosphate Synthase (DUMPS) are autosomal recessive inherited diseases in Holstein cattle breed. The aim of this study was to investigate whether FXI defi ciency and DUMPS alleles exist in Holstein cattle reared in Antalya region Turkey or not. 504 Holstein cows were used for this study. PCR (Polymerase Chain Reaction) was used for determination ...

The cancellation level and phase were measured for the (f2-f1) distortion product in six normal-hearing ears as a function of input level (L1,L2) and the frequency separation of the two input signals (f1,f2, where f2 greater than f1). The effects of input level were examined for L1 and L2, varied together (L1 = L2) and separately. Typically, f1 was 1500 Hz while f2/f1 was either 1.16, 1.32, 1.4...

The purpose of the research was to analyze results a change in service period, yaliiness and reduction losses using new protocol for prevention gynecological diseases, as well mathematical calculation forecast changes coefficient life-lifetime reproduction cows.Materials methods. work performed on 215 cows Holstetskaya breed Leningrad type. To prevent occurrence diseases afternoon complications...

*Corresponding Author Rajesh. K. Patel [email protected] The present study investigated the occurrence of an autosomal recessive genetic disease, Bovine Citrullinaemia caused by mutation in Argininosuccinate Synthase (ASS) gene, in Indian Holstein cattle. The Polymerase chain reactionRestriction fragment length polymorphism (PCR-RFLP) analysis was performed on a group of 120 Holstein bulls to...

Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection. Samples of 331 anima...

Induction of twinning by ipsilateral nonsurgical transfer of two frozen-thawed Japanese Black bovine embryos to each of 20 Holstein and 26 Japanese Black cows, that had been kept under a stable in private farm conditions, was examined. The cows were monitored every 20 days from Day 2.5 to Day 65 of gestation for pregnancy and fetus survival (estrus is Day 0). Seventy-five per cent ( 1.5 of 20),...

A study was conducted to evaluate the genetic variation in bitter ground using SNP (single-nucleotide polymorphism) markers. Thirty -five primers showing reliable polymorphism were used .This paper mainly applies molecular directive from self-absorbed population of F6 Cho Moi/Ben Tre. The Polymorphism on two directives, TP1386 and TP 1877 with Bitter gourd LG1. wide observed for morphological t...

Recombinant Der f2 (rDer f2) has recently been developed as a promising allergen for the diagnosis and immunotherapy of house-dust mite allergy, and studies in immunology. The aim of the present study was to evaluate whether oral administration of rDer f2 could suppress an immediate allergic reaction in mice sensitized with mite allergen. We developed a murine allergic model that showed broncho...