The structure-function relationship of interferons (IFNs) has been studied by epitope mapping. Epitopes of bovine IFNs, however, are practically unknown, despite their importance in virus infections and in the maternal recognition of pregnancy. It has been shown that recombinant bovine (rBo)IFN-alphaC and rBoIFN-alpha1 differ only in 12 amino acids and that the F12 monoclonal antibody (mAb) bin...

Shiga-toxigenic Escherichia coli strains belonging to serotype O157 are important human pathogens, but the genetic basis of expression of the O157 antigen and the role played by the lipopolysaccharide O side chain in the adherence of this organism to epithelial cells are not understood. We performed TnphoA mutagenesis on E. coli O157:H7 strain 86-24 to identify a mutant (strain F12) deficient i...

Article history: Received on: 13/08/2014 Revised on: 09/09/2014 Accepted on: 15/10/2014 Available online: 27/11/2014 Desloratadine (DS) is a tricyclic antihistaminic, characterized by bitter taste and slight water solubility. The aim of this study is to prepare DS as orally disintegrating tablets (ODT) to mask the bitter taste and improve compliance. Twelve different placebo ODT (F1-F12) were p...

Phosphatidylinositol phosphates are important regulators of processes such as the cytoskeleton organization, membrane trafficking and gene transcription, which are all crucial for polarized cell growth. In particular, phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] has essential roles in polarized growth as well as in cellular responses to stress. In the yeast Saccharomyces cerevisiae, th...

High-level ab initio calculations on the ground state X1Σ+ and the excited state A1Π of the BH molecule were carried out by using the explicitly correlated multireference configuration interaction method (MRCI-F12) plus Davidson correction (+Q) and scalar relativistic correction (+SR). The potential energy curves (PECs) of the X1Σ+ and A1Π states were obtained. From the computed PECs, the spect...

Explicitly correlated local coupled-cluster (LCCSD-F12) methods with pair natural orbitals (PNOs), orbital specific virtual orbitals (OSVs), and projected atomic orbitals (PAOs) are compared. In all cases pair-specific virtual subspaces (domains) are used, and the convergence of the correlation energy as a function of the domain sizes is studied. Furthermore, the performance of the methods for ...

The fundamental vibrational frequency of the B–N stretch in BH3NH3 has eluded gas-phase experimental observation for decades. This work offers a theoretical anharmonic prediction this mode to be 644 cm−1, using Cartesian quartic force field at CCSD(T)-F12/cc-pVTZ-F12 level theory. other frequencies reported herein have mean absolute error only 5 cm−1 from seven available frequencies, making and...

A detailed description of the explicitly correlated second-order Møller-Plesset perturbation theory (MP2-F12) method, as implemented in the TURBOMOLE program package, is presented. The TURBOMOLE implementation makes use of density fitting, which greatly reduces the prefactor for integral evaluation. Methods are available for the treatment of ground states of open- and closed-shell species, usin...

Speaker independent discrimination of four confusable consonants in the strictly fixed context of six vowels is considered. The consonants are depicted by features of consonant’s stationary part and changing rate of features (delta features) in transition from consonant to the following vowel. The mel frequency cepstrum (MFCC), linear prediction cepstrum (LPCC), recursive filter (F12) features ...