D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) is the first committed enzyme of L-serine biosynthesis in the phosphorylated pathway. To determine the physiological importance of Phgdh-dependent L-serine biosynthesis in vivo, we generated Phgdh-deficient mice using targeted gene disruption in embryonic stem cells. The absence of Phgdh led to a drastic reduction of L-serine metabolites s...

PURPOSE To understand the difference between macular and peripheral regions, tissue samples of human retina, retinal pigment epithelium, and Bruch's membrane/choroid were dissected and analyzed for lipid composition. METHOD To facilitate dissections and enhance the recovery of tissues, eyecups were prefixed for 1 hour in 10% formalin (pH 7). Lipids were extracted from 4-mm trephined punches o...

Effects of ethanol treatment on Leydig cell NADPH-generating enzymes and lipid profiles were studied. Ethanol treatment (3.0 g/kg. b.wt.) twice daily as a 25% (v/v) aqueous solution given to adult Wistar rats reduced the body weight, testis weight and relative weights of the seminal vesicles and ventral prostate. Serum LH and testosterone were also decreased. Similarly, the NADPH-generating enz...

Article history: Received on: 25/05/2016 Revised on: 17/06/2016 Accepted on: 10/07/2016 Available online: 30/08/2016 Phospholipid is very essential in the balanced diet. The vegetarian people in the coastal area are habitant of using edible oil seeds as daily food grains. Salinity of water during cultivation decreases the accumulation of oil content (12-15%) in seeds. Present experiment was foc...

introduction: crude vegetable oils contain some quantities of phospholipids. the highest concentration of phospholipids is usually found in crude soybean oil. the presence of phospholipids in the crude and refined oils create undesirable effects on the quality and quantity of the oil during refining operations or use by the consumer. however, if phospholipids are extracted properly from the oil...

BACKGROUND Recurrent pregnancy loss (RPL) has been associated with antiphospholipid antibodies (APA) including anticardiolipin and lupus anticoagulant. Therapy using heparin and aspirin has been shown to significantly improve the live birth rate. We evaluated whether other APA should be considered as a basis for treatment in women with RPL. We also assessed the efficacy of heparin and aspirin t...

Slein, Milton W. (Fort Detrick, Frederick, Md.), and Gerald F. Logan, Jr. Characterization of the phospholipases of Bacillus cereus and their effects on erythrocytes, bone, and kidney cells. J. Bacteriol. 90:69-81. 1965.-Culture filtrates of Bacillus cereus contain phospholipases that split phosphoryl choline, phosphoryl ethanolamine, and phosphoryl inositol from the phospholipids phosphatidyl ...