The present study deals with the establishment of rolA-transgenic and rolB-transgenic plants for first time through Agrobacterium tumefaciens mediated transformation, exploiting inherent morphogenic potential an important medicinal plant, Bacopa monnieri (L.) Wettst. showed integration expression rolA rolB genes respectively, whereas Ri-transformed rolA, rolB, rolC rolD genes. Morphogenic diffe...

Organ-culture studies were made using cartilage from achondroplastic (dwarf) rabbits (ac/ac) and their phenotypically normal litter-mates. A significantly higher incorporation of C from glucose and galactose was measured in the dwarf; incorporation of S from sulfate and H from thymidine was equal in the two types of explant. Also, CO2 and [ C]lactate production from glucose by the dwarf cartila...

Explants of leaves, petals, anthers, styles and ovaries of C. speciosus, C. cancellatus, C. gilanicus and C. almehensis were cultured on LS media with three different combinations of NAA and BAP in the dark. Callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. Corm explants from these species were also cultured on...

Regeneration of explants plays a significant role in plant transformation. Explant type, hormonal concentration, and pre-culturing period are important in transformation efficiency. To get an efficient transformation of canola and optimize regeneration conditions, different explants along with different culture media were studied. Four canola varieties were used to evaluate regeneration ability...

PURPOSE This study investigates the effects of heat shock treatment and the role of Hsp70 and Hsp90 on tranforming growth factor beta 2 (TGF-beta2)-induced epithelial-to-mesenchymal transition (EMT) in rat lens epithelial explants. METHODS Rat lens epithelial explants from 7 to 10 day-old Wistar rats were dissected and incubated for 24 h before treatment. The explants were divided into eight ...

Rumex thyrsiflorus Fingerh. is one of the few dioecious plant species, which have sex chromosomes. We conducted the preliminary experiments to determine the type of morphogenesis of R. thyrsiflorus explants cultured in vitro and to verify, using PCR-based methods, if there is the relationship between sex and morphogenetic response of explants micropropagated under in vitro conditions. The resul...

While the UV-MITF pathway is well-established as a key player of pigmentation, implication other MITF independent pathways in this biological process still unclear. Illustrating this, several evidence demonstrated role oxidative stress pigmentation disorders but exact mechanisms by which potentially impacts skin are incompletely elucidated. Here, we identified conserved mechanism demonstrating ...

In order to enhance the system of potato transformation and further regeneration, four potato cultivars were transformed using the Agrobacterium tumefaciens harboring â-glucuronidase (GUS) gene.The results revealed that the highest percentages of shoot formation obtained were for Spunta cv. Stem explants resulted in more callus and regeneration after transformation and selection than leaf expla...