The alveolar epithelium is a dynamic tissue, consisting of cells types I and II, covering more than 99 % the lung inner surface actively responding to various endogenous exogenous stimuli. technology alveolocyte isolation presented, which consists in mechanical disaggregation tissue with subsequent processing resulting explants 0.25 trypsin solution combination filtration cell suspension throug...

Antibiotic-resistant bacterial infections represent an emerging health concern in clinical settings, and a lack of novel developments in the pharmaceutical pipeline is creating a "perfect storm" for multidrug-resistant bacterial infections. Antimicrobial peptides (AMPs) have been suggested as future therapeutics for these drug-resistant bacteria, since they have potent broad-spectrum activity, ...

Surfactant deficiency at birth makes it difficult for the newborn to inflate its lungs. As the infant makes increasingly vigorous attempts to ventilate noncompliant lungs, delayed adsorption of lung fluid, pulmonary edema, extravasation of plasma proteins into air spaces, and lung injury occur, which cause progressive respiratory distress. Intratracheal administration of surfactant into the inf...

Exogenous or inhaled NO (iNO) has been successfully used, as a selective pulmonary vasodilator, in a wide variety of clinical situations especially in the management of persistent pulmonary hypertension in the newborn. A better understanding of the role of endogenous and exogenous NO in the lungs of surfactant-deficient animals exposed to hyperoxia could result in novel strategies for the bette...

PURPOSE Exogenous surfactant is not very effective in adults with ARDS, since surfactant does not reach atelectatic alveoli. Perfluorocarbons (PFC) can recruit atelectatic areas but do not replace impaired endogenous surfactant. A surfactant-PFC-mixture could combine benefits of both therapies. The aim of the proof-of-principal-study was to produce a PFC-in-surfactant emulsion (Persurf) and to ...

one of the main components of lung alveoli is surfactant. dppc (dipalmitolphosphatidylcholine) is thepredominant lipid component in lung surfactant that is responsible for lowering surface tension in alveoli in thisarticle. we used a very approximate model with computational method of ab initio to describe the interactionsbetween dppc as important component of lung surfactant and some chemical ...

One pharmaceutical preparation of exogenous lung surfactant, SURFACEN, was tested in vitro on growth of pathogens involved in lung disease. SURFACEN at concentration of 1, 10 and 20 mg/mL was incubated with Streptococcus pneumoniae, Streptococcus agalactiae, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli in saline solution by 5 h at 37 °C. In the presence of SURFACEN it was o...

Targeted deletion of the surfactant protein (SP)-B locus in mice causes lethal neonatal respiratory distress. To assess the importance of SP-B for postnatal lung function, compound transgenic mice were generated in which the mouse SP-B cDNA was conditionally expressed under control of exogenous doxycycline in SP-B-/- mice. Doxycycline-regulated expression of SP-B fully corrected lung function i...