The effect of 30-day dark deprivation (light-dark cycle 24/0 h, artificial illumination 300 Lux) on the morphofunctional state testes was studied using morphological and morphometric analysis histological preparations white mongrel male rats 4 months age. level follicle-stimulating hormone in blood serum assessed by enzyme immunoassay. It found that increases serum. Morphological changes were c...

The objectives of this study were to (1) determine the effect exogenous fibrolytic enzyme derived from Trichoderma reesei on dry matter (DM) and neutral detergent fibre (NDF) degradability whole plant faba bean silage (Snowbird), (2) evaluate effects (FETR) DM NDF degradation kinetics silage, (3) compare difference between in vitro approach (DaisyII incubation method) situ assay-biological (nyl...

The aim of the present study was to characterize the acyl-coenzyme A: cholesterol acyltransferase (ACAT) activity in human liver microsomes. Liver biopsies were obtained from patients undergoing elective cholecystectomy under highly standardized conditions. In 34 patients the enzyme activity of the microsomal fraction averaged 6.6 +/- 0.7 (mean +/- SEM) pmol.min-1.mg protein-1 in the absence of...

Although many investigations have been carried out on the n-amino acid oxidase since its discovery in 1935 (2)) the metabolic role of this enzyme remains somewhat obscure. It is generally believed, on the basis of indirect evidence, that in animals the enzyme functions in the inversion of exogenous n-amino acids over the pathway n-amino acid --+ Lu-keto acid -+ L-amino acid (3). The first step ...

Inherited deficiency of the housekeeping enzyme triosephosphate isomerase (TPI) is the most severe clinical disorder of glycolysis. Homozygotes manifest congenital hemolytic anemia and progressive neuromuscular impairment, which in most cases pursues an inexorable course with fatal outcome in early childhood. No effective therapy is available. Hitherto specific enzyme replacement has not been a...

Although many investigations have been carried out on the n-amino acid oxidase since its discovery in 1935 (2)) the metabolic role of this enzyme remains somewhat obscure. It is generally believed, on the basis of indirect evidence, that in animals the enzyme functions in the inversion of exogenous n-amino acids over the pathway n-amino acid --+ Lu-keto acid -+ L-amino acid (3). The first step ...

In Chenopodium album, leaf excision and light both increase urease (EC. 3.5.1.5) activity. Dithiothreitol (DTT), reduced glutathione (GSH), cysteine and diazoinedicarboxylic acid bis(N,N-dimethylamide) (diamide) activated the crude enzyme. In contrast, crude urease was inhibited by phenylmethylsulphonyl fluoride (PMSF) and Na-p -tosyl-L-lysine chloromethyl ketone HCl (TLCK), suggesting the pres...

Melatonin is a new plant hormone that plays an important role in stress tolerance. For investigation the effect of exogenous application of melatonin on salt tolerance in rosemary (Rosmarinus officinalis L.), a factorial experiment was conducted in a completely randomized design with three replications. The first factor was melatonin (50, 100 µM) and the second factor was salinity stress (6, 9,...

Using methyl-1-thio-beta-D-galactoside as the inducer, the biosynthesis of beta-galactosidase was observed in Escherichia coli B with only endogenous sources of nitrogen and energy available. The addition of glucose, ribose, xylose, or glycerol as exogenous energy sources to nitrogen-deficient media blocked enzyme formation. Preinduction of the resting cells failed to overcome inhibition by the...