Eucalyptus gunnii Hook microcuttings, obtained in vitro through axillary bud stimulation, show different rooting responses on the same rooting medium depending on the physiological state induced by cytokinins used in the previous multiplication medium. 6-Furfurylamino purine and 6-(4-hydroxy-3-methylbut-2-enylamino)purine induced a physiological state characterized by high sensitivity of microc...

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost ...

Agar substrates for in vitro culture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted for in vitro culture and functional root production. To reinforce this hypothesis, this study compares in vitro development of nemesia on several substrates. Strong differences between nemesia ro...

Shoots of apple rootstocks raised in vitro were transferred to various rooting media to study the effect of different factors on root initiation and development. Various concentrations of indole-3-butyric acid (IBA) initiated rooting but maximum rooting percentage was found with 2.0 and 2.5 mg l(-1) of IBA in M7 and with 1.0 mg l(-1) of IBA in MM106. The drawback was that the roots were thick, ...

An efficient protocol for in vitro clonal propagation of Smilax zeylanica was developed using nodal and shoot tip segments from field grown plants. Nodal explants performed better than when two distinct were tested proliferation. The best results proliferation achieved with MS medium supplemented BAP Kn alone or combination NAA IBA at various concentrations. On containing 4.0 ?M BAP, the greate...

The influence of two carbon sources, sorbitol and sucrose on In vitro shoot proliferation and rooting of peach rootstock GF 677 was compared at 15, 30, 45 and 60 g l concentrations. The highest number of shoots per proliferated explant, usable shoots and shoots having maximum fresh weight were obtained on sorbitol at 30 g l. GF 677 does not seem to be an efficient utilizer of sucrose for In vit...

tissue culture methods provide tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, in vitro propagation of diaphanoptera khorasanica rech.f., a rare and threatened plant species with limited distribution range and population was investigated. this species has a potential as an ornamental plant. single node explants wer...