The agents daunomycin, ethidium bromide, distamycin A and cytochrome c inhibit DNA dependent DNA polymerase I (E. coli) reaction competitively to DNA. The influence of these template inactivators on the binding of DNA polymerase to native as well as denatured DNA has been determined by affinity chromatography. Cytochrome c blocks the binding of the enzyme to double-stranded and to single-strand...

In organello labelling of Trypanosoma brucei mitochondrial (mt) RNA was characterised with respect to nucleotide requirements and drug sensitivity. Mitochondrial transcriptional activity is maximal in the presence of all ribonucleoside-triphosphate NTPs, and can be inhibited by UTP depletion. Mitochondrial transcription can also be partially inhibited by actinomycin D (actD) or ethidium bromide...

The fluorescent dye ethidium bromide stains Escherichia coli and Staphylococcus aureus in whole blood. The staining is rapid, relatively specific, and does not require fixation of the sample. Furthermore, stained bacteria can be seen microscopically without the need for a final wash to remove unbound dye. By using lysostaphin, an S. aureus-specific lytic enzyme, we have demonstrated that S. aur...

HeLa S3 cells were synchronized by selective mitotic detachment and chromatin was isolated from synchronized populations at various times during the cell cycle. The isolated chromatins were studied by circular dichroism and for their ability to bind the intercalating dye, ethidium bromide. Chromatin from cells in the middle of the S phase showed an increase in maximum ellipticity in the 250- to...

The DNA polymerase enzymes from avian, murine, and feline RNA tumor viruses can be distinguished by their ability to read specific, synthetic primertemplates. The copying of templates containing adenylic and thymidylic acids by all these DNA polymerases is inhibited by ethidium bromide, though this compound affects the polymerases from mammalian tumor viruses much more than the enzyme from avia...

Exonuclease III has been shown previously to reveal the binding sites of the antitumor drug cis-diamminedichloroplatinum(II) on DNA. Pretreatment of the same DNA with the intercalator ethidium bromide causes new platinum binding sites to be detected by the exonuclease III method. In particular, a 5'd(G6-D-G2)3' sequence in a 165-base-pair restriction fragment of plasmid pBR322 becomes a preferr...