BACKGROUND Microorganisms can adapt to perturbations of the surrounding environment to grow. To analyze the adaptation process of the yeast Saccharomyces cerevisiae to a high ethanol concentration, repetitive cultivation was performed with a stepwise increase in the ethanol concentration in the culture medium. METHODOLOGY/PRINCIPAL FINDINGS First, a laboratory strain of S. cerevisiae was cult...

Abstract Bioethanol production has been a challenge for the researchers with respect to enhancing bioethanol yield. In this study, we are reporting an efficient novel method produce bioethanol. The process comprises co-culture technique from wheat straw, by co-culturing Bacillus licheniformis and Saccharomyces cerevisiae. Simultaneous saccharification fermentation allows straw hydrolysis cellul...

UNLABELLED In the model eukaryote Saccharomyces cerevisiae, the transcription factor Cst6p has been reported to play important roles in several biological processes. However, the genome-wide targets of Cst6p and its physiological functions remain unknown. Here, we mapped the genome-wide binding sites of Cst6p at high resolution. Cst6p binds to the promoter regions of 59 genes with various biolo...

The early death of two non-Saccharomyces wine strains (H. guilliermondii and H. uvarum) during mixed fermentations with S. cerevisiae was studied under enological growth conditions. Several microvinifications were performed in synthetic grape juice, either with single non-Saccharomyces or with mixed S. cerevisiae/non-Saccharomyces inocula. In all mixed cultures, non-Saccharomyces yeasts grew to...

BACKGROUND Saccharomyces cerevisiae strains expressing D-xylose isomerase (XI) produce some of the highest reported ethanol yields from D-xylose. Unfortunately, most bacterial XIs that have been expressed in S. cerevisiae are either not functional, require additional strain modification, or have low affinity for D-xylose. This study analyzed several XIs from rumen and intestinal microorganisms ...

Furfural produced during lignocellulose pretreatment to reduce the recalcitrance inhibits growth of Saccharomyces cerevisiae and reduces ethanol yield. To adverse effect furfural on S. cerevisiae, exogenous ergosterol was supplemented impact under stress studied. The lag phage shortened by 50%, maximum yield increased 158% with 50 mg/L supplementation 4 g/L stress. Flow cytometry analysis resul...

BACKGROUND Efficient xylose fermentation by yeast would improve the economical and sustainable nature of biofuels production from lignocellulosic biomass. However, the efficiency of xylose fermentation by the yeast Saccharomyces cerevisiae is suboptimal, especially in conversion yield, despite decades of research. Here, we present an improved performance of S. cerevisiae in xylose fermentation ...

BACKGROUND Starch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. Cost-effective utilisation of starch requires consolidated bioprocessing (CBP) where a single microorganism can produce the enzymes required for hydrolysis of starch, and also convert the glucose monomers to ethanol. RESULTS The Aspergillus tubing...

Ethanol production from lignocellulosic biomasses raises a global interest because it represents a good alternative to petroleum-derived energies and reduces the food versus fuel conflict generated by first generation ethanol. In this study, alkaline-acid pretreated brewers’ spent grain (BSG) was evaluated for ethanol production after enzymatic hydrolysis with commercial enzymes. The obtained h...

in this study, phenolic compounds of evening primrose leaves (oenothera biennis) were extracted with aceton 70%, ethanol 70% and methanol 70%. total phenolic content of aceton extract was significantly higher than the ethanolic and methanolic extracts. extracts were tested for their antimicrobial activity against lactobacillis plantarum and saccharomyces cerevisiae, using micro broth dilution m...