background and purpose: brain-derived neurotrophic factor (bdnf) is present in the hippocampus and cerebral cortex in high concentrations and has an important role in development, maintenance and regeneration of the nervous system. the aim of this study was to produce active bdnf in e.coli using transforming plasmid. methods: to construct an expression plasmid of bdnf, a gene coding for it was ...

the present study was conducted to investigate antimicrobial resistant pattern of escherichia coli (e. coli) strains isolated from clinical specimens of jordanian pediatric patients during the period from january to december 2008. a total of 444 e. coli strains were isolated from clinical specimens and tested for their susceptibility to different antimicrobial drugs. overall, high resistance ra...

background and objectives: bacteria need iron for growth and most of them can actively acquire fe ions using especial iron-chelating proteins which named siderophores. we aimed to determine the frequencies of iuca, iron and irp2 genes in the uropathogenic escherichia coli (upec) isolates. we also analyzed the effects of siderophore genes beside iron supplements on growth rate of the isolates. m...

background: the emergence and propagation of different phylogenetic groups of antimicrobial-resistant e. coli have become a worldwide health concern in human and veterinary medicine. therefore, the evaluation of the phylogenetic distribution of antibiotic-resistant e. coli is important for therapeutic and economic purposes. the aims of this study were to determine phylogenetic groups and patter...

background and objectives: the therapeutic options for diseases caused by escherichia coli are limited. in this study we investigated the presence of virulence factors among enteropathogenic escherichia coli (epec) strains and their antibiotic resistance patterns. the isolates were also checked for the presence of class1 integrons and gene cassettes. materials and methods: this study included 7...

conclusions the analysis recommended that ta genes are prevalent in clinical isolates of e. coli strains. the analysis revealed that hipba ta system is associated with biofilm formation. results the results of the analysis revealed that 107 e. coli isolates were potent for biofilm formation by cra. the findings by microtiter plates showed that 102 e. coli isolates were biofilm producers. the re...

conclusions the alcoholic extract of p. harmala was very effective against the selected esbl-producing e. coli isolates harboring the tem gene. therefore, it could be suggested as an antibacterial agent in the future. more researches are necessary for detecting the mechanism of this plant’s behavior and its pharmacological effects. results results of this study showed that in disk diffusion met...

conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...

conclusions anova results showed that the effect of cycle and time on the selected bacterial removal efficiency were more important than that of the microbial concentration. results the results showed that e. coli and e. faecalis were effectively treated at 10 pulse/s in 9 minutes and 6 log cfu/ml bacterial suspension (p < 0.0001 for e. coli with 99.99% (4 log) and p = 0.0002 for e. faecalis wi...

background genetic manipulation of chloroplast in higher plants offers a number of unique prerogatives, including; undesirable of pleiotropic genome and gene silencing effects and also use as an important agronomic trait for producing essential biomaterials and industrial enzymes. in order to manipulate chloroplast genome, specific vectors are required. these vectors can be transformed and expr...