conclusions the alcoholic extract of p. harmala was very effective against the selected esbl-producing e. coli isolates harboring the tem gene. therefore, it could be suggested as an antibacterial agent in the future. more researches are necessary for detecting the mechanism of this plant’s behavior and its pharmacological effects. results results of this study showed that in disk diffusion met...

conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...

conclusions anova results showed that the effect of cycle and time on the selected bacterial removal efficiency were more important than that of the microbial concentration. results the results showed that e. coli and e. faecalis were effectively treated at 10 pulse/s in 9 minutes and 6 log cfu/ml bacterial suspension (p < 0.0001 for e. coli with 99.99% (4 log) and p = 0.0002 for e. faecalis wi...

background genetic manipulation of chloroplast in higher plants offers a number of unique prerogatives, including; undesirable of pleiotropic genome and gene silencing effects and also use as an important agronomic trait for producing essential biomaterials and industrial enzymes. in order to manipulate chloroplast genome, specific vectors are required. these vectors can be transformed and expr...

aim : we aimed to develop a multiplex pcr assay for specific detection of epec and ehec pathotypes based on specific marker genes. background : about 2.5 million infant’s morbidity in developing countries occurs by e.coli pathotypes because of diarrhea and intestinal diseases. the traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are...

conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...

conclusions our study indicated a high frequency of cotrimoxazole resistance gene in e. coli isolates from ilam and tehran (milad) hospitals, and sul genes had a major role in cotrimoxazole resistance of these isolates. materials and methods one-hundred and forty-four e. coli isolates were identified during march 2007 to april 2012 at ilam hospitals and milad (tehran) hospital. antibiotic susce...

conclusions the β-lactamase disk test is appropriately sensitive to detect esbls in mdr isolates of e. coli. background most urinary tract infections (utis) are caused by escherichia coli (e. coli) species. due to infections outbreaks of e. coli strains with multiple mechanisms of resistance to β-lactam antibiotics, the sensitivity of confirmatory tests to detect the extended spectrum β-lactama...

background: escherichia coli is one of the most important pathogens which could be transmitted by milk. the main aim of the present study was to assess risk factors related to the occurrence of e. coli in fluid milk marketed in chittagong, bangladesh. methods: totally, 169 milk samples were collected from different entry points in chittagong, bangladesh; and also, 17 samples were directly colle...

in this study, 90 internal organ samples of poultry with symptoms of colibacillosis were obtained from maragheh poultry farms in east azerbaijan, iran. in total, 70 bacterial isolates were confirmed as escherichia coli (e. coli) strains using standard biochemical tests, and antibiotic sensitivity was determined by the disk diffusion method. antibiotics used in this study included ampicillin, pe...