Havel, R. J., Eder, H. A. & Bragdon, J. H. (1955) J. Clin. Invest. 34, 1345-1353 Henly, A. A. (1957) Analyst (London) 82,286-287 Kane, J. P. (1973) Anal. Eiochern. 53, 35e364 Lowry, 0. H.,Rosebrough, N. J., Farr, A. L. &Randall, R. J. (1951)J. Eiol. Chem. 193,265-275 Mies, 0. D., Faergeman, O., Hamilton, R. L. & Havel, R. J. (1975)J. Clin. Invest. 56,603-615 Noel, S . P., Dolphin, P. J. & Ruben...

Various derivatives of adenosine were prepared by acylation of adenosine (6-amino-9-(bD-ribofuranosyl)purine (1) with different molar equivalents of acetic anhydride and/or pivaloyl chloride in pyridine. Compounds 6-acetylamino-9-[(2,3,5-tri-O-acetyl)-b-Dribofuranosyl]purine (3), 6-amino-9-[(2,3,5-tri-O-acetyl)-b-D-ribofuranosyl]purine (4), and 6-pivaloylamino-9-[(2,3,5-tri-O-pivaloyl)-b-D-ribo...

Competitive inhibition as a function of pH for the metalloendoprotease thermolysin by derivatives of L-alpha-(2-hydroxyphenyl)benzenepropanoyl-L- tryptophanylglycylglycine exhibits a diagnostic bell shape. Binding is maximal between two pKa values: on the acidic limb the apparent Ki value is regulated by an unchanging enzymic ionization (pKa 5.3) which is also seen in the substrate-hydrolysis k...

Sphingomyelin in mixed dispersion with bile salts was hydrolysed by the solubilized sphingomyelinase of rat brain lysosomes. In parallel studies, physical properties of these dispersions were determined. The kinetic curves that described the rate of hydrolysis as a function of increasing concentrations of bile salt were multiphasic. A region of very low activity was followed by an ascending por...