Abstract Cheese-making involves milk coagulation as a crucial step where calf rennet has been used traditionally the milk-clotting enzyme in cheese industry. This study investigated enzymes from seven species of seaweed and evaluated parameters for their isolation partial purification including post-harvest processing, extraction methods. The saturation degree ammonium sulphate precipitation st...

Flavor development in ripening Cheddar cheese depends on complex microbial and biochemical processes that are difficult to study in natural cheese. Thus, our group has developed Cheddar cheese extract (CCE) as a model system to study these processes. In previous work, we found that CCE supported growth of Lactobacillus casei, one of the most prominent nonstarter lactic acid bacteria (NSLAB) spe...

The objective of this collaborative study was to determine interlaboratory performance statistics for a modified and optimized version of AOAC Method 920.123 for the determination of the total nitrogen content of hard, semihard, and processed cheese by Kjeldahl analysis. Details included addressing the issues of material homogeneity, test portion size (1 g), quantitative transfer (weighing on t...

Aroma compounds and sensory features of lactose free (LFM) traditional (TM) Mozzarella cheese have been investigated during their labeled shelf-life. Acetoin 2-heptanone characterized both types at the production time. During shelf-life, a statistically significant increase in amount volatiles coming from amino acid fatty metabolism occurred LFM samples after 8 days storage and, to lesser exten...

This work aimed at evaluating the influence of enzyme concentration, temperature, and reaction time in the lactose hydrolysis process in milk, cheese whey, and whey permeate, using two commercial β-galactosidases of microbial origins. We used Aspergillus oryzae (at temperatures of 10 and 55°C) and Kluyveromyces lactis (at temperatures of 10 and 37°C) β-galactosidases, both in 3, 6, and 9 U/mL c...

Little information is available on the growth and activity of bacteria at temperatures above the optimum, despite the fact that in many commercial processes the organisms used are often subject to temperatures higher than those most favorable for growth. In the manufacture of Swiss cheese, for example, starter cultures must not only survive the high temperatures used during cooking of the curd,...

This study was conducted to determine the AFM1 levels in Egyptian raw milk, soft cheese and AFB1 in table eggs marketed in Kafrelsheikh city markets. Aflatoxin content was measured using microtiter-plate enzyme-linked immunosorbent assay (ELISA). The Test Kits, RIDASCREEN ELISA of Biopharm AG that used is based on competitive enzyme immunoassay for the quantitative analysis of Aflatoxin AFM1 an...

This work deals with the development of a new bioprocess for the efficient synthesis of lactosucrose, a potential prebiotic oligosaccharide with a high value-added, from two important and inexpensive agro-industrial by-products such as tofu whey and cheese whey permeate. The bioconversion is driven by the ability of the enzyme levansucrase SacB from Bacillus subtilis CECT 39 to transfructosylat...

The range of sodium chloride (salt)-to-moisture ratio is critical in producing high-quality cheese products. The salt-to-moisture ratio has numerous effects on cheese quality, including controlling water activity (a(w)). Therefore, when attempting to decrease the sodium content of natural cheese it is important to calculate the amount of replacement salts necessary to create the same a(w) as th...