b ackground : tuberculosis is a crucial health problem. establishing a rapid, reliable and still inexpensive diagnostic method for tuberculosis seems to be substantial in developing countries where tb has very high incidence rate . methods:   an indirect  enzyme-linked  immunosorbent  assay (elisa )   was established to detect serum antibodies against mycobacterium  tuberculosis. three kinds of...

biochemical characteristics of α-amylase in the digestive system and head glands of cryptolaemus montrouzieri, a key predator of citrus mealybug, planococcus citri (pseudococcidae), were studied. the major isoform of α-amylase with the same molecular weight was detected in both gut and head glands loaded on polyacrylamide-starch gel electrophoresis. moreover, a minor band with much lower intens...

conclusions afm1 contamination of milk is not a concern in this city. however, dairy cattle feed samples of various livestocks must be regularly checked for aflatoxin and the storage conditions of feeds should be strictly controlled. results afm1 was detected in all milk samples. the mean concentration of afm1 was 11.61 ± 0.72 ng/l with a range of 6.3-23.3 ng/l. none of the samples had levels e...

The main objective of this study was to purify and characterize an esterase from Shewanella sp F88. The enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and Q-sepharore chromatography. Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. The enzyme showed an optimum activity at pH 6.5 and 58 ˚C. Evo...

Antibodies directed against the enzyme glutamic acid decarboxylase (GAD) are believed to be the main cause of destruction of pancreatic islet cells in type I (insulin dependent) diabetes mellitus. The enzyme was found both in the brain and pancreatic beta cells. Although similarities in identity of GAD in human and rat brain have been demonstrated, little is known about the interaction betw...

the effects of different storage temperatures and times on germination rate, malondialdehyde (mda), superoxide dismutase (sod), peroxidase (pod) and catalase (cat) activities in jatropha curcas l. seed were investigated. the results showed that germination rates of seeds stored at room temperature and 4°c for 1 month was significantly higher than those stored for 3 months at -20 and -80°c. mda ...

an experiment was conducted to study the effects of enzyme and phytogenic product (thymol+carvacrol) on the ileal microbial population and jejuna and ileal histomorphology of broilers fed wheat based diet. the experiment was conducted as a 2 × 3 factorial arrangement with 2 levels of enzyme endofeed w (0 and 0.05%) and 3 levels of next enhance 150® (0, 100 and 200 mg/kg). each of the 6 dietary ...

the presence of microbial load and high fiber content in various non conventional feedstuffs limit their utilization in poultry feed. in the present study, the feedstuffs were treated with acidified sodium chlorite (asc) and its impact on  the microbial load and metabolizable energy availability was assessed in  the chickens. the effect of supplementation of feed grade enzyme was also evaluated...

an enzyme electrode was developed using the enzyme sulfite oxidase (ec1.8.3.1) immobilized onto pig intestine. hydrogen peroxide produced was monitored amperometrically. all experimental parameters such as ph, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. the response to sulfite concentration was linear in the range 5.2´10-6 to 1.02´...

background: recently, applications of nanoparticles in many fields of medicine have been developed, due to their specific physical and chemical properties. therefore assessment of their toxicity specially in the in vivo condition is necessary. objectives: the aim of this study is to evaluation the effect of fe3o4 nanoparticles coating by biocompatible compounds on their toxicity and also compar...