materials and methods the expression of hyaluronidase antigenic fragments was optimized using iptg concentration, time of induction, temperature, culture, and absorbance of 0.6-0.8-1 at 600 nm. afterwards, the expressed proteins were purified and the enzymatic activity was assessed by turbid metric method. results data indicated that maximum protein is produced in od = 0.8, 0.5 mm isopropyl β-d...

thermoplastic starch (tps) materials present several advantages to the plastic industry andwhen blended with other materials they can exhibit improved mechanical and moisturesensitivity properties compared to pure tps materials. further investigations on tps: pvablends are of particular interest due to their excellent compatibility and improved properties suchas tensile strength, elongation, to...

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewat...

in order to estimate the reducing sugar in tubers of potato by using a manual glucose determiner, an experiment was conducted in factorial arrangement based on completely randomized design with three replications. in this research reducing sugar of four cultivars of potato – agria, sante, with two methods: enzymatic and a manual glucose determiner. the results picaso and kizer – that maintained...