Zinc is known to be an essential element for human nutrition and good health1). Milk is its sole source in infants, and growing children as well as mature adults consume varying amounts of milk, which provide them with much of the needed zinc. Investigators, using gel filtration chromatography2,3), have demonstrated that zinc in cow's milk can be associated with fractions of high molecular weig...

The results of Klee and Richards (1) are in disagreement with the conclusions of Anfmsen et al. (2-4) that concentrated urea has no effect on the activity of bovine pancreatic ribonuclease. The possibility exists that differences in enzymatic activity might be also caused by alteration of the substrate rather than of the enzyme upon treatment with these agents. In examining the effect of condit...

Identification of recalcitrant factors that limit digestion of forages and the development of enzymatic approaches that improve hydrolysis could play a key role in improving the efficiency of meat and milk production in ruminants. Enzyme fingerprinting of barley silage fed to heifers and total tract indigestible fibre residue (TIFR) collected from feces was used to identify cell wall components...

Laminarin, a water-soluble polysaccharide is known to induce aggregation and fusion of liposomes. Using laminarin specific lysing enzymes for multi-lamellar liposomes their aggregation was found to be fully reversible, in contrast to small unilamellar liposomes for which irreversible fusion is the main process. Moreover, our results indicate that these processes are probably mediated by the for...

Liquid hot (LHW) water pretreatment (LHW) of lignocellulosic material enhances enzymatic conversion of cellulose to glucose by solubilizing hemicellulose fraction of the biomass, while leaving the cellulose more reactive and accessible to cellulase enzymes. Within the range of pretreatment conditions tested in this study, the optimized LHW pretreatment conditions for a 15% (wt/vol) slurry of hy...

Chronic wasting disease (CWD) and sheep scrapie can be transmitted via indirect environmental routes, and it is known that soil can serve as a reservoir of prion infectivity. Given the strong interaction between the prion protein (PrP) and soil, we hypothesized that binding to soil enhances prion resistance to enzymatic digestion, thereby facilitating prion longevity in the environment and prov...

S A M U E L E . S A U N D E R S , † J A S O N C . B A R T Z , ‡ K U R T C . V E R C A U T E R E N , § A N D S H A N N O N L . B A R T E L T H U N T * , † Department of Civil Engineering, Peter Kiewit Institute, University of NebraskasLincoln, Omaha, Nebraska 68588, Department of Medical Microbiology and Immunology, Creighton University, Omaha, Nebraska 68178, and USDA Animal and Plant Health In...

Probiotics are nonpathogenic microorganisms that, when ingested, exert a positive influence on the health or physiology of the host. They can influence intestinal physiology either directly or indirectly through modulation of the endogenous ecosystem or immune system. The results that have been shown with a sufficient level of proof to enable probiotics to be used as treatments for gastrointest...

We report a new and general approach that will be useful for adapting the method of electrocatalytic amplification (ECA) to biosensing applications. In ECA, individual collisions of catalytic nanoparticles with a noncatalytic electrode surface lead to bursts of current. In the work described here, the current arises from catalytic electrooxidation of N2H4 at the surface of platinum nanoparticle...