Molecular Enzymology Group Colloquium Organized by D. Fell (School of Biological and Molecular Sciences, Oxford Brooke University) and D. Kell (Department of Biological Sciences, University College, Wales, Aberystwyth), Edited by D. Fell and Sponsored by Xenova Ltd, Zeneca Bioproducts, Glaxo Research and Development Ltd and SmithKline Beecham Pharmaceuticals. 653rd Meeting held at the Universit...

Accurate, economical and high-throughput gene and genome synthesis is essential to the development of synthetic biology and biotechnology. New large-scale gene synthesis methods harnessing the power of DNA microchips have recently been demonstrated. Yet, the technology is still compromised by a high occurrence of errors in the synthesized products. These errors still require substantial effort ...

Introd u ction Clinical chemistry, for decades restricted to a limited number of chemical tests, has, in recent years, through an increasing awareness of the value of the laboratory investigation of disease, and the introduc­ tion of automation and sophisticated instru­ mentation, expanded its field of operations to include a bewildering variety of tests. Advances in biochemical knowledge have ...

Trideuteriated trichothecenes can be generated from diacetoxyscirpenol by a reaction sequence involving acetylation, epoxide deoxygenation, allylic oxidation, deuterium exchange, epoxidation, and reduction.

Palm oil is a promising raw material for biofuel production using the simultaneous catalytic mechanism of bifunctional cracking-deoxygenation reactions. Through process, chains palmitic acid and oleic in palm were converted to diesel-range hydrocarbons. The combination effects CaCO3 HY zeolite enhanced into biofuel, because increasing basic sites catalysts due synergistic roles HY. introduction...

The effects of erythrocyte aggregation on O(2) release were examined using O(2)-permeable fluorinated ethylenepropylene copolymer tubes (inner diameter, 25 microm; outer diameter, 100 microm). Measurements were performed using an apparatus built on an inverted microscope that contained a scanning-grating spectrophotometer with a photon count detector connected to two photomultipliers and an ima...

The concentration of dissolved oxygen in aquatic systems helps to regulate biodiversity1,2, nutrient biogeochemistry3, greenhouse gas emissions4, and the quality drinking water5. long-term declines concentrations coastal ocean waters have been linked climate warming human activity6,7, but little is known about changes lakes. Although solubility decreases with increasing water temperatures, lake...

Ischemic preconditioning (IPC) improves maximal exercise performance. However, the potential mechanism(s) underlying the beneficial effects of IPC remain unknown. The dynamics of pulmonary oxygen uptake (VO2) and muscle deoxygenation during exercise is frequently used for assessing O2 supply and extraction. Thus, this study examined the effects of IPC on systemic and local O2 dynamics during th...

In order to define the influence of skeletal protein organization on transmembrane phospholipid movement in erythrocyte membranes, we measured the translocation rate of lysophosphatidylcholine in pathologic red cells. A simple method based on the differential extraction of lysophosphatidylcholine from the red cell membrane by saline and albumin solutions was used to quantitate the translocation...

The oxygenation state of erythrocytes is known to impact several cellular processes. As the only known O2-binding protein in red blood cells, haemoglobin has been implicated in the oxygenation-mediated control of cell pathways and properties. Band 3, an integral membrane protein linked to the spectrin/actin cytoskeleton, preferentially binds deoxygenated haemoglobin at its N-terminus, and has b...