Chlamydia trachomatis is an obligate intracellular parasite of eucaryotic cells. Little is known about the role of the host in supporting chlamydial replication beyond the facts that host cells provide ATP and that de novo host protein synthesis is not required for bacterial growth. To further explore potential contributions of host nuclear function to chlamydial development, we questioned whet...

In cells that had been physically enucleated after treatment with cytochalasin B (cytoplasts) levels of cholesterol synthesis and 3-hydroxy-3-methylglutaryl coenzyme A reductase activity were nearly constant over a 6-h period of time. The ratio of the inactive to the active form of the reductase was unaltered by enucleation and did not change when the cytoplasts were incubated at 37%. The addit...

Enucleated human polymorphonuclear leukocytes (PMN) were prepared by centrifuging isolated, intact PMN over a discontinuous Ficoll gradient that contained 20 microM cytochalasin B. The enucleated cells (PMN cytoplasts) contained about one-third of the plasma membrane and about one-half of the cytoplasm present in intact PMN. The PMN cytoplasts contained no nucleus and hardly any granules. The v...