One hundred and thirty two Bacillus cereus and 52 Bacillus megaterium isolates from honeys were evaluated for the presence of genes encoding enterotoxin HBL, enterotoxin-T, cytotoxin K and the NHE complex, respectively. The relationship between hemolytic and coagulase activity and its correlation with the presence of the four mentioned enterotoxins was determined by principal component analysis...

Although opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis...

INTRODUCTION Staphylococcal species are pathogens that are responsible for outbreaks of foodborne diseases. The aim of this study was to investigate the prevalence of enterotoxin-genes and the antimicrobial resistance profile in staphylococcus coagulase-negative (CoNS) and coagulasepositive (CoPS) isolates from black pudding in southern Brazil. METHODS Two hundred typical and atypical colonie...

BACKGROUND AND OBJECTIVES Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also important. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastit...

Abstract Staphylococcus aureus is an important foodborne pathogen associated to food intoxication and other multiple infections in human being. Its presence salted a serious issue due its salt tolerance potential. A study was conducted analyze the of enterotoxins producing drug resistance S. sea fish from Gwadar. Freshly persevered samples (n=50) were subjected using 16S rRNA Nuc genes primers....

Abstract Staphylococcal enterotoxins (SE) are essential in human and animal infection food poisoning. This study analyzed ten genes ( sek, sel, sem, sen, seo, sep, seq, ser, ses , set ) encoding new staphylococcal enterotoxin isolated from animals humans by multiplex polymerase chain reaction (PCR). In Yogyakarta, Indonesia, samples cases (174 isolates), cattle (5 goats isolates) resulted a tot...

VYLETĚLOVÁ, M., BANYKÓ, J.: Detection of coding genes for enterotoxins in Bacillus cereus by PCR and their products by BCET-RPLA and ELISA Assay. Acta univ. agric. et silvic. Mendel. Brun., 2010, LVIII, No. 5, pp. 417–426 Determination of enterotoxin production, diarrhoeal and emetic gene identifi cation was studied in 41 Bacillus cereus strains isolated from raw cows’ and raw goats’ milk, past...

Abstract The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli the leading agents in food poisoning. present work aims to evaluate microbiological quality cheeses commercialized Brazil, regarding microbiology safety enterotoxin gene presence. Forty-three samples from five different suppliers were analyzed. Counting identification aureus, Salmonella spp perform...

BACKGROUND/AIM Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nar...

Hemolysin BL (HBL) is the multi-component enterotoxin causing diarrheal type of Bacillus cereus food poisoning. The hbl operon of B. cereus was divided into 4 coding sequences (CDS) and identified as hblC, hblD, hblA and hblB genes consisting of 1,319, 1,221, 1,128 and 1,401 nucleotides encoding proteins of 439, 406, 375 and 466 amino acids with predicted sizes of 49.3, 43.8, 41.7 and 52.3 kDa,...