Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcus faecalis strain OG1RF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OG1RF and TX5179 strains were tested in a mixed-infection (inoculum, approximatel...

The methyl moiety of ribothymidine in the tRNA of Streptococcus faecalis, Bacillus subtilis, and probably Bacillus cereus is dervied from a 1-carbon folate derivative and not S-adenosylmethionine. Micrococcus luteus (M. lysodeikticus) tRNA appears to be almost devoid of ribothymidine. S. faecalis tRNA lacking ribothymidine can be methylated in vitro with formation of ribothymidine. 5,10-Methyle...

Six new ent-kaurane diterpene glycosides, pharbosides A-F (1-6), and a new ent-gibbane diterpene glycoside, pharboside G (7), together with three known ent-kaurane diterpenoids, 7beta,16beta,17-trihydroxy-ent-kauran-6alpha,19-olide (8), 6beta,7beta,16alpha,17-tetrahydroxy-ent-kauranoic acid (9), and 6beta,7beta,16beta,17-tetrahydroxy-ent-kauranoic acid (10), were isolated from an ethanolic extr...

1 MD by the Federal University of Ceará (Resident Physician at the ENT Service of the Walter Cantídio University Hospital of the Medical School of the Federal University of Ceará). 2 PhD in Medicine by the Medical School of the University of São Paulo (Adjunct Professor in the ENT Course of the Medical School of the Federal University of Ceará). 3 PhD in Medicine by the Federal University of Ce...

We studied the influences of antimicrobial agents on the colonization of the respiratory tract and infection with Enterococcus faecalis in intensive care unit (ICU) patients receiving mechanical respiration for at least 3 days. In a matched-cohort analysis, patients receiving topical antimicrobial prophylaxis (TAP) of the oropharynx and stomach with antimicrobial agents not treating E. faecalis...

Endoscopes have become an indispensable instrument in the ENT department, but their use has introduced potential health risks such as the infection transmission. Numerous guidelines have been issued for both digestive and respiratory endoscopes, while to date specific references to ENT endoscopes do not exist. The diagnostic ENT endoscope does not generally have an operative channel, it is shor...

Aim: the aim of the present ExVivo study was to check the antimicrobial efficacy of silver nanoparticles with and without different antimicrobials against E. faecalis. Material and Methods: 126 recently extracted single rooted human teeth were contaminated with E. faecalis. The teeth were randomly divided into 5 experimental (n=21) and 1 control group (n=21). Each subgroup was then exposed to d...

BACKGROUND High-level aminoglycoside resistance (HLAR) that precludes bactericidal synergism with penicillins or glycopeptides and nephrotoxicity related to aminoglycoside treatment are major problems in treating Enterococcus faecalis endocarditis. OBJECTIVE To evaluate the efficacy and safety of ampicillin plus ceftriaxone for treating endocarditis due to E. faecalis with and without HLAR. ...

Interleukin-12 (IL-12) is an important cytokine for the immunomodulatory effects of lactic acid bacteria (LAB). Using murine immune cells, we previously reported that the RNA of Enterococcus faecalis EC-12, a LAB strain exerting probiotic-like beneficial effects, is the major IL-12-inducing immunogenic component. However, it was recently revealed that bacterial RNA can be a ligand for Toll-like...

OBJECTIVE The aims of this study were to investigate the presence of Enterococcus faecalis in primary endodontic infections and failed endodontic treatments using real-time PCR and to determine the statistical importance of the presence of E. faecalis in a Turkish population with endodontic infections. MATERIAL AND METHODS E. faecalis was investigated from 79 microbial samples collected from ...