Gibberellins (GAs) are involved in the regulation of numerous developmental processes in plants including zygotic embryogenesis, but their biosynthesis and role during somatic embryogenesis (SE) is mostly unknown. In this study we show that during three week- long induction phase, when cells of leaf explants from non-embryogenic genotype (M9) and embryogenic variant (M9-10a) were forming the ca...

St Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is an important warm season turf and pasture grass. In vitro tissue culture of St Augustinegrass could serve as an important mean for its improvement through genetic transformation as well as induced somaclonal variation. To optimize tissue culture conditions for plant regeneration of St Augustinegrass, tissue culture responses of 11 ex...

optimization of regeneration methods in cotton is necessary to improve and successful production of transgenic cotton cultivars by tissue culture technique. this study was conducted to determine the genotypic effect on callus induction in cotton using 3 genotypes (coker349, 4-s-4 and c1211) and coker 312 selected and a study was carried out in cotton research institute of gorgan using a factori...

Rye is an important crop in Northern and Eastern Europe. However, the application of various biotechnologies in rye breeding has been limited duo to its recalcitrant in tissue culture. In order to improve somatic tissue effi ciency, key factors affecting somatic embryogenesis and reproducible green plant regeneration of rye (Secale cereale L.) were evaluated and optimised. In this study, a tota...

While the progress made in vitro culture of Chinese fir has produced satisfactory results, further improvements are warranted. To understand mechanism somatic embryogenesis (SE) fir, we conducted phenotypic observations, physiological and biochemical measurements, transcriptome analysis embryonic (EC) non-embryogenic callus (NEC) to provide a scientific basis for SE this species. We found that ...

Embryogenic tissue culture systems are utilized in propagation and genetic engineering of crop plants, but applications are limited by genotype-dependent culture response. To date, few genes necessary for embryogenic callus formation have been identified or characterized. The goal of this research was to enhance our understanding of gene expression during maize embryogenic tissue culture initia...

The objectives of this work were to evaluate in vitro response of Iranian alfalfa cultivars and select high regenerative genotypes. Somatic embryogenesis response of leaf and petiole explants from five alfalfa cultivars and landraces was studied using six regeneration protocols. Cultivars and landraces exhibited protocol, and explant-specific response and somatic embryo formation occurred only ...

This long-term study demonstrates for the first time that it is possible to propagate embryogenic cultures in pelargoniums and to subsequently initiate the differentiation of embryos using the cultivar Madame Layal (Pelargonium x domesticum). Propagation of callus was only possible with combinations of 2,4dichlorophenoxyacetic acid (2,4-D) and 6benzylaminopurine (BAP), which gave rise to embryo...