ABSTRACT: Bovine viral diarrhea virus (BVDV) is a highly infectious pathogen that affects bovines worldwide leading to great economic impact. Although Brazil has the largest commercial cattle population throughout world and an increasing buffalo breeding industry, country no control or eradication program for BVDV. In this perspective, aim of study was evaluate occurrence BVDV in buffaloes from...

BACKGROUND Hepatitis G virus (HGV) is a member of Flaviviridae. Prevalence of HGV in healthy people is very low, but this virus is more prevalent in patients with hepatitis. Besides, relative frequency of HGV in patients undergoing hemodialysis, and kidney recipients is very high. The role of HGV in pathogenesis is not clear. Since this virus cannot be cultivated, molecular techniques such as R...

BACKGROUND & OBJECTIVES Diagnosis of dengue infection in acute phase is important for clinical care, implementing control measures, surveillance and research. Currently, dengue fever is diagnosed by means of virus isolation, reverse transcriptase PCR or IgM and IgG based ELISA. Given the limitations of all the existing diagnostic methods, there is a need for rapid, sensitive and high throughput...

Avian leukosis retroviruses (ALV) cause lymphomas and other cancers in chickens. Previous studies have used enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) to detect ALV p27 group-specific antigens (GSA) in commercial chicken eggs. In the poultry industry eradication programme against exogenous ALV, ELISA assays are used to identify chickens infected with...

BACKGROUND Early and rapid detection of dengue virus (DENV) infection during the febrile period is crucial for proper patient management and prevention of disease spread. An easy to perform and highly sensitive method is needed for routine implementation especially in the resource-limited rural healthcare settings where dengue is endemic. METHODS A single-tube reverse transcription-loop-media...

Dengue is a serious tropical disease caused by the mosquito-borne dengue virus (DENV). Performant, rapid, and easy-to-use assays are needed for accurate diagnosis of acute DENV infection. We evaluated performance three prototype developed VIDAS® automated platform to detect NS1 antigen anti-dengue IgM IgG antibodies. Positive negative agreement with competitor enzyme-linked immunosorbent (ELISA...

MX virus is a Snow Mountain agent (SMA) genogroup human calicivirus (HuCV) identified in a Mexican child with diarrhoea. An ELISA using hyperimmune antisera to the recombinant MX virus (rMX) capsid was developed to detect SMA genogroup HuCVs in stool specimens. The rMX ELISA detected the prototype MX virus, SMA, and Hawaii agent (HA), but not Norwalk virus (NV) or Sapporo virus. Twenty-three di...

Early definitive diagnosis of dengue virus infection may help in the timely management of dengue virus infection. We evaluated the Standard Diagnostics (SD, South Korea) dengue virus nonstructural protein NS1 antigen enzyme-linked immunosorbent assay (SD dengue NS1 Ag ELISA) for the detection of dengue virus NS1 antigen in patients' sera, using a total of 399 serum samples in a comparison with ...

BACKGROUND AFRIMS longitudinal dengue surveillance in Thailand depends on the nested RT-PCR and the dengue IgM/IgG ELISA. OBJECTIVE To examine and improve the sensitivity of the nested RT-PCR using a panel of archived samples collected during dengue surveillance. STUDY DESIGN A retrospective analysis of 16,454 dengue IgM/IgG ELISA positive cases collected between 2000 and 2013 was done to i...

background: to investigate the expression of tnf-α, il-6, il-10 in the plasma of patients with lung cancer who received radiation therapy (rt), and to analyze the correlation between these cytokines and radiation pneumonitis (rp). materials and methods: patients with lung cancer who received 3d-crt in our hospital were prospectively evaluated. circulating cytokine levels were measured with elis...