نتایج جستجو برای: electroporation
تعداد نتایج: 4856 فیلتر نتایج به سال:
Electroporation is a next generation bioelectronics device. The emerging application of electroporation requires high voltage pulses having pulse-width in the nanosecond range. essential use capacitor results an increase size electroporator circuit. This paper discusses modification conventional Marx generator circuit to achieve with minimal chip reduced capacitors are attributed reduction numb...
Bioimpedance measurements are used to determine physiological aspects of biological tissues. On the other hand, the electroporation phenomenon causes a variation in the electrical properties of tissue, so it is possible use bioimpedance measurement with the aim of monitor the electroporation phenomenon in real time. The objective of this article is present the basic concepts required to underst...
Electroporation is the application of electric pulses of sufficient amplitude to target tissue, which entails not only permeabilization of cell membranes, but also heat generation and dissipation. Noticeable rises in temperature have been observed in a number of electroporation applications. These temperature rises are a potential source of alteration of thermodynamic properties of tissue where...
Exposing biological cells to sufficiently strong external electric fields causes electroporation of cell membranes, i.e. occurrence of transient or permanent permeable pathways between the interior and exterior of the cell. Electroporation can be used to introduce various molecules into cells (reversible electroporation) or to kill cells (irreversible electroporation), which can in turn be used...
Electroporation has been widely used to load impermeant exogenous molecules into cells. Rapid electrical lysis based on electroporation has also been applied to analyze intracellular materials at single-cell level. There has been increasing demand to implement electroporation in a microfluidic format as a basic tool for applications ranging from screening of drugs and genes to studies of intrac...
Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with un...
Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.
Abstract It is quite necessary to develop a safe and efficient technique for disinfection of drinking water avoid waterborne pathogens infectious diseases. Herein, ZnO nanoarray electrodes with different sizes were investigated low-voltage high-efficiency electroporation disinfection. The results indicated that the nano-pyramid small tip width proper length exhibited over 99.9% efficiency again...
Skin electroporation has recently been shown to increase transdermal transport of small-size drugs as well as considerably larger molecules by up to 4 orders of magnitude in vitro. Nevertheless, no in vivo studies have proven that high-voltage pulses can induce therapeutic plasma levels of drug. The aim of the present report was precisely to study the potential of skin electroporation in transd...
The transdermal delivery of drugs and, in more general terms, active principles can be performed by iontophoresis and/or electroporation. advantages a local treatment vs. other intake methods are well known as it reduces the systemic involvement and therefore contraindications. To perform exploiting electroporation effect there is nowadays specific variant medical device used physiotherapy: Med...
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