The murine monoclonal antibody (MAb) DF3 was prepared against a human breast carcinoma. Previous studies have demonstrated that DF3 antigen levels are elevated in plasma of patients with breast cancer. Furthermore, MAb DF3 reacts with circulating glycoproteins of different molecular weights ranging from approximately 300 to 450 kd. The present study demonstrates that plasma DF3 antigen is compr...

Experiments are described on the electrophoretic mobility of a single isolated colloid and the electroosmotic response of the surrounding medium. For that optical tweezers are employed which enable one to trap a particle without any mechanical contact and to measure its position and the forces acting on it with high resolution (±2 nm, ±200 fN). In a custom-made microfluidic cell, the two effect...

Over the past 10 years, fluorescent end-labeling of DNA fragments has evolved into the preferred method of DNA detection for a wide variety of applications, including DNA sequencing and PCR fragment analysis. One of the advantages inherent in fluorescent detection methods is the ability to perform multi-color analyses. Unfortunately, labeling DNA fragments with different fluorescent tags genera...

This paper presents the features of electrophoretic deposition TiO2, TiO2-$$$MWCNT composites. The influence various dispersant additives in suspension on mobility TiO2 particles and morphology formed layer has been studied. effect modification by Au Cu clusters photocatalytic activity was investigated.

Zeta potential is a physicochemical parameter of particular importance in describing ion adsorption and double layer interactions between charged particles. However, for metal-oxide nanoparticles, the conversion of electrophoretic mobility measurements into zeta potentials is difficult. This is due to their very high surface electrical conductivity, which is inversely proportional to the size o...

Arthrobotrysamerospora (ATCC 34468) produced glucoamylase in a semi-synthetic medium containing starch as a sole carbon source. Polyacrylamide gel electrophoresis of crude glucoamylase showed three isoenzymes. They were designated as glu I, glu II and glu III according to their electrophoretic mobility. These iso-glucoamylases were purified by column chromatography using DEAE-Sephadex A-50. The...

The electrophoretic mobility shift assay (EMSA), or gel mobility shift assay, is a popular and powerful technique for the detection of RNA-protein interactions. It relies on the fact that naked RNA has certain mobility on nondenaturing gels, but if the RNA is bound by protein, the mobility of the RNA is reduced. Therefore, the binding of protein results in a characteristic upward shift of the R...

The molecules occurring as terminal residues on the external surfaces of nuclei prepared from rat liver by either sucrose-CaC12 or citric acid methods and nucleoli derived from the sucrose-CaC1 2 nuclei were studied chemically and electrokinetically . In 0.0145 M NaCl, 4.50/,) sorbitol, and 0 .6 mM NaHCO3 with pH 7 .2 t 0.1 at 25°C, the sucrose-CaC12 nuclei had an electrophoretic mobility of -1...