A technique for assay of lecithinase antibodies in serum was developed in this laboratory by using a lecithin-agar plate diffusion procedure based on a combination of described plate assays. Egg yolk lipoprotein composed primarily of lecithin was used as a substrate for reaction with free or non-neutralized lecithinase C after incubation of known amounts of lecithinase C with various dilutions ...

Pure phosphatidyl ethanolamine and lecithin from egg yolks were fed to rats in saline or in olive oil and the changes in individual phospholipids in the intestinal wall, liver, and plasma of the animals were studied. Ingestion of olive oil alone produced increased levels of all phospholipid fractions in each of the three tissues. Feeding phosphatidyl ethanolamine in saline resulted in slightly ...

Pig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in b...

2014 Slightly hydrated egg lecithin was aligned mostly parallel to the glass plates of the sample cells by squeezing it to a thickness of 20 03BCm. The rest of the cell was filled with water and the lipid was left to swell at elevated temperatures to bilayer mean spacings between 3 and 17 nm. Cooling by a few °C gave rise to induced adhesion in the semicylindrical border (and myelin cylinders) ...

This work is a continuation of earlier research concerning the influence of tin compounds on the dynamic properties of liposome membranes produced with lecithin hen egg yolks (EYL). The experiments were carried out at room temperature (about 25 degrees C). Four tin compounds were chosen, including three organic ones, (CH3)4Sn, (C2H5)4Sn and (C3H7)3SnCl, and one inorganic, SnCl2. The investigate...

Entomopathogenic nematodes are soil-dwelling biocontrol agents. EPNs need an insect host to complete their life cycle, and they kill during its development. The major disadvantage of is the high cost commercial products. Thus, there many studies focused on reducing production costs by optimization mass production. In a previous project, Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: He...

Celiac disease is a serious and lifelong disorder that associated with gluten consumption. patients should commit to strict free diet. Besides celiac patients, can also cause allergenic reactions in significant portion of population. Thus, there growing trend replacing sources alternatives. This work concentrated on improving the quality consumer acceptance gluten-free cakes made out rice flour...

There are still concerns over sperm quality when plant-based diluents used instead of animal-based semen extenders. Therefore, in our study we compared the effects one soybean lecithin-based (AndroMed®) and two egg yolk-based (BullXcell® Optidyl®) commercially available extenders on post-thaw vitro quality. Fifty ejaculates collected from ten bulls were aliquoted into three parts diluted with a...

When model membranes are prepared by ultrasonic treatment of polyunsaturated phospholipids, radical production can induce a partial degradation of the polyunsaturated fatty acyl chains and the formation of lipid hydroperoxides. A suitable antioxidant employed during liposome preparation is able to protect them against lipid peroxidation. This work contains the results of studies on egg lecithin...

Knowledge of the molecular packing of lipids and water in lipid bilayers is important for understanding bilayer mechanics and thermodynamics. Information on packing is most often obtained from x-ray or neutron diffraction measurements. Given the d spacing, composition, and partial specific volumes of the lipid and water, it is a simple matter to calculate the area per lipid molecule, bilayer th...