We developed a new simple enzymatic assay method for measuring urinary polyamines (total amount of putrescine, spermidine, and cadaverine), using an acylpolyamine amidohydrolase and a putrescine oxidase. First conjugated polyamines (putrescine, spermidine, and cadaverine) in urine were hydrolyzed by incubation with an acylpolyamine amidohydrolase at 30 degrees for 1 hr. then, free polyamines we...

The interaction between the 15-mer oligonucleotide d[A(TA)(7)].d[T(AT)(7)] and the three biogenic polyamines, putrescine, spermidine and spermine, under physiological conditions has been studied by Raman spectroscopy. Solutions containing 60 mM (phosphate) of the oligonucleotide and different polyamine concentrations ranging from 1 to 75 mM have been studied. Both natural and heavy water were u...

Polyamines play an important role in the maintenance of intestinal permeability. Therefore we aimed to determine effects probiotics and omega 3 fatty acids on serum polyamine levels colitis. Fifty BALB/c mice were randomly grouped as normal, colitis with no treatment applied, treated by (VSL#3), omega-3, both omega-3. Experimental was induced injection 200 mg/kg 2,4-Dinitrobenzenesulfonic acid ...

Polyamine synthesis and accumulation was studied in several species of developing sea urchins. Most striking is the presence in the gametes of a large amount of spermine, with low amounts of putrescine and spermidine. This contrasts with the pattern of polyamines present in both micro-organisms and mammals. Micro-organisms contain mainly putrescine and spermidine and adult mammalian tissues usu...

It was previously reported that bacteriophage T4 contains the polyamines putrescine, NH2(CH2)$;IH2, and spermidine, NHQ(CH&NH(CH&NH2, in amounts sufficient to neutralize about half of the viral deoxyribonucleic acid (1). The putrescine and spermidine in the phage were found to be derived from the large amount of these polyamines normally present in the host bacterium, Escherichia coli B. It was...

The urinary excretion of the polyamines--putrescine, spermidine, and spermine--was measured in 7 children with Beckwith's syndrome. Putrescine excretion was raised and spermidine excretion reduced. The raised putrescine and the low spermidine ratios were highly significant. These results are consistent with a disturbance in a metabolic pathway under growth hormone-like regulation.

S-(5'-Deoxy-5'-adenosyl)methylthioethylhydroxylamine (AMA) is an irreversible inhibitor of S-adenosylmethionine (AdoMet) decarboxylase, which is designed to bind covalently the pyruvate residue at the enzyme active site. In the present study the cellular effects of AMA were characterized for the first time in cultured L1210 leukaemia cells. At the approximate IC50 (concn. giving 50% inhibition;...

Putrescine, spermidine, and spermine are a group of small organic cations, collectively known as polyamines. They are present in all living cells, and their levels are generally increased in tumor cells. Progesterone receptor is a gene-regulatory protein that plays a major role in gestation and in hormonal responsiveness of breast cancer. We studied the effects of putrescine, spermidine, 2 lowe...

Ethylene initiates the ripening and senescence of climacteric fruit, whereas polyamines have been considered as senescence inhibitors. Ethylene and polyamine biosynthetic pathways share S-adenosylmethionine as a common intermediate. The effects of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception, on ethylene and polyamine metabolism and associated gene expression was investigat...

The growth of BHK-21/C13 cells in monolayer cultures was inhibited by 6-thioguanosine. Accumulation of putrescine, spermidine, and spermine was inhibited by 6-thioguanosine, and cells incubated in the presence of the drug had a decreased content of polyamines relative to control cells. These effects were more marked for spermidine than for spermine or putrescine. Consequently, the intracellular...