background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

Associations between house dust-associated beta-(1,3)-glucan exposure and airway inflammatory reactions have been reported, while such exposures in early childhood have been suggested to protect against asthma and wheezing. Most epidemiological studies have used reservoir dust samples and an inhibition enzyme immunoassay (EIA) for beta-(1,3)-glucan exposure assessment. The objective of this stu...

Florida Department of Transportation (FDOT) sponsored research performed at the University of Florida resulted in developing a wireless monitoring and real time static capacity estimate technology for driven piles. This new technology, Embedded Data Collector (EDC), uses two levels of instrumentation, embedded in the body of precast prestressed concrete piles near the head and tip. Strain and a...

Corneal disease has become the second leading cause of blindness in world. transplantation is currently considered to be one common treatments for vision loss. This paper presents a novel approach utilizing dual-crosslinked membranes composed polyrotaxane multiple aldehydes (PRAs), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimide (NHS) development process. Collagen...

(cSBL) is known to have anti-tumor activity. cSBL is a multifunctional protein and is recognized as a pyrimidine basespecific ribonuclease. In respect to its ribonuclease activity, cSBL belongs to the RNase A superfamily, and is a very heat-stable enzyme. It retains 100% activity after heating at 100 °C for 5 min. Four RNases have been isolated from the Rana family of frogs, and their primary s...

background : in this study, the inhibitory effect of ethylene dichloride (edc) on biodegradation was investigated using the specific methanogenic activity (sma) test of anaerobic biomass. methods: in the present study, 40 sma tests were performed using 120-ml vials in batch mode. substrate, biomass, and biogas occupied 66%, 17%, and 17% (v/v) of the volume of each vial, respectively. the durati...