BACKGROUND The halophilic bacterium Chromohalobacter salexigens has been proposed as promising cell factory for the production of the compatible solutes ectoine and hydroxyectoine. This bacterium has evolved metabolic adaptations to efficiently grow under high salt concentrations by accumulating ectoines as compatible solutes. However, metabolic overflow, which is a major drawback for the effic...

The aim of this investigation was to perform an extensive screening using HPLC and I3C-NMR spectroscopy to disclose the spectrum of osmolytes produced by aerobic heterotrophic and anoxygenic phototrophic eubacteria. The most predominant solutes detected within a wide range of marine and halophilic micro-organisms were two recently discovered tetrahydropyrimidines ectoine and hydroxyectoine, whi...

The synthesis of the compatible solute ectoine, mediated by the ectABC gene products, is the main mechanism used by the halophilic bacterium Chromohalobacter salexigens to cope with osmotic stress. Evidence was found that this process is regulated at the transcriptional level. S1 protection analyses performed with RNA extracted from cells grown in minimal medium at low (0.75 M NaCl) or high (2....

Bacillus ligninesis strain L1, isolated from seafloor sediment, was able to grow on medium with lignin as its sole carbon source. Here, we report a 3.8-Mbp high-quality genome sequence for this bacterium. The genes involving ectoine and glycine betaine synthesis, as well as those involved in the degradation of lignin, were identified.

Vibrio cholerae is both an intestinal pathogen and a microbe in the estuarine community. To persist in the estuarine environment, V. cholerae must adjust to changes in ionic composition and osmolarity. These changes in the aquatic environment have been correlated with cholera epidemics. In this work, we study the response of V. cholerae to increases in environmental osmolarity. Optimal growth o...

BACKGROUND Halophilic bacteria have shown their significance in industrial production of polyhydroxyalkanoates (PHA) and are gaining more attention for genetic engineering modification. Yet, little information on the genomics and PHA related genes from halophilic bacteria have been disclosed so far. RESULTS The draft genome of moderately halophilic bacterium, Halomonas sp. TD01, a strain of g...

Process conditions for the synthesis and subsequent secretion of ectoine and hydroxyectoine were optimized in a cascade of two continuously operated bioreactors. In the first bioreactor the halophile bacterium Chromohalobacter salexigens was grown under continuous hyperosmotic and thermophilic conditions and therefore under constant stress driving the cells to accumulate large amounts of ectoin...

The increased use of ectoine and hydroxyectoine in cosmetics pharmaceuticals has led to the increasing demand for these bioactive compounds. This study aims investigate production by halophilic bacteria isolated from traditional solar saltern at Pejarakan Village, Buleleng Regency, Province Bali, Indonesia. were grown brine sediment samples using Luria Bertani media. was determined inoculating ...