As part of our studies of the class II genes of miniature swine, we have isolated and characterized cDNA clones corresponding to DRB genes from two major histocompatibility complex homozygous strains. Comparison of the sequences of these clones to those of human DRB genes revealed a striking amino acid homology between the hypervariable residues of SLA-DRBc and the human DRB1-0101 allele. The p...

Measurements of nitric oxide (NO) pulmonary diffusing capacity (DL(NO)) multiplied by alveolar NO partial pressure (PA(NO)) provide values for alveolar NO production (VA(NO)). We evaluated applying a rapidly responding chemiluminescent NO analyzer to measure DL(NO) during a single, constant exhalation (Dex(NO)) or by rebreathing (Drb(NO)). With the use of an initial inspiration of 5-10 parts/mi...

Neoplastic mouse lung epithelial cells contain greatly diminished activity, protein, and mRNA for the type I isozyme of cyclic AMPdependent protein kinase (PKA I), while expression of the type II isozyme (PKA II) is similar to that of normal lung cells. A time course of PKA mRNA content in transcriptionally inhibited cells indicated that most PKA mRNAs are more stable in the neoplastic E9 cell ...

The major histocompatibility complex (MHC) presents a unique system to explore links between genetic diversity and pathogens, as diversity within MHC is maintained in part by pathogen driven selection. While the majority of wildlife MHC studies have investigated species that are of conservation concern, here we characterize MHC variation in a common and broadly distributed species, the North Am...

Discarded red beetroot (DRB) is an organic waste generated in the food industry. This study focused on valorization of DRB through recovery bioactive compounds. The characterization juice confirmed a higher content antioxidants (DPPH 504 ± 24 μmol TE/L, FRAP 10920 440 ABTS 22012 592 TE/L), phenolic compounds (1789 56 mg GAE/L), flavonoids (471 17 CE/L) and betalains (1426 mg/L) than commercial ...

Climate change affects the hydrological cycle of river basins and strongly impacts water resource availability. The mechanistic model PROMET was driven with an ensemble EURO-CORDEX regional climate projections under emission scenarios RCP2.6 RCP8.5 to analyze changes in temperature, precipitation, soil content, plant stress, snow equivalent (SWE) runoff dynamics Danube River Basin (DRB) near (2...

Defatted rice bran (DRB) is a low-cost, high-fiber, antioxidant-rich byproduct of oil production that has limited application in food systems due to its undesirable impacts on sensory attributes. This research aimed determine the feasibility integrating defatted into tomato salad dressing without undergoing chemical or enzymatic pretreatment. The DRB particle size (38, 90, and 150 µm) concentra...

Salicylic acid (SA) activates immediate early transcription of genes controlled by a family of DNA promoter elements named as-1-like elements. These elements are functional in the promoter of glutathione S-transferase genes. We have previously shown that SA increases the binding of tobacco (Nicotiana tabacum cv Xanthi nc) nuclear factors to the as-1 sequence in a process mediated by protein pho...

The acute effect of RNA and DNA synthesis inhibitors on DNA topoisomerase (topo) I localization within cells was examined. Indirect immunofluorescence revealed that topo I was distributed throughout the nuclei but was concentrated in nucleoli of untreated K562 leukemia cells and A549 non-small cell lung cancer cells. Treatment with the DNA polymerase inhibitor aphidicolin did not alter this dis...

The acute effect of RNA and DNA synthesis inhibitors on DNA topoisomerase (topo) I localization within cells was examined. Indirect imiminofluorescence revealed that topo I was distributed throughout the nuclei hut was concentrated in nucleoli of untreated K562 leukemia cells and A549 non-small cell lung cancer cells. Treatment with the DNA polymerase inhibitor aphidicolin did not alter this di...